Antibody Name:	Anti-Rad18 Antibody
Antibody SKU:	CAB13434
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_064550.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, MCF7, Jurkat, 293T, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human Rad18 (NP_064550.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	INKH LDSC LSRE EKKE SLRS SVHK RKPL PKTV YNLL SDRD LKKK LKEH GLSI QGNK QQLI KRHQ EFVH MYNA QCDA LHPK SAAE IVRE IENI EKTR MRLE ASKL NESV MVFT KDQT EKEI DEIH SKYR KKHK SEFQ LLVD QARK GYKK IAGM SQKT VTIT KEDE STEK LSSV CMGQ EDNM TSVT NHFS QSKL DSPE ELEP DREE DSSS CIDI QEVL SSSE SDSC NSSS SDII RDLL EEEE AWEA SHKN DLQD TEIS PRQN RRTR AAES AEIE PRNK RNRN
Gene ID:	56852
Uniprot:	Q9NS91
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	56kDa
Observed MW:	80kDa

Synonyms:

RAD18, RNF73

Background:

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

UniProt Protein Function:	RAD18: a structural maintenance of chromosomes (SMC) protein involved in postreplication repair of UV-damaged DNA. Has ssDNA binding activity. Interacts with Rad6 through a conserved ring-finger motif. Rad6 is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA.
UniProt Protein Details:	Protein type:DNA repair, damage; EC 6.3.2.19; Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 3p25.3 Cellular Component: XY body; replication fork; nucleus; chromatin Molecular Function:Y-form DNA binding; protein binding; zinc ion binding; ubiquitin protein ligase binding; damaged DNA binding; polyubiquitin binding; ligase activity Biological Process: negative regulation of DNA recombination; protein ubiquitination; spermatogenesis; DNA repair; response to UV
NCBI Summary:	The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NS91
NCBI GenInfo Identifier:	313104165
NCBI Gene ID:	56852
NCBI Accession:	Q9NS91.2
UniProt Secondary Accession:	Q9NS91,Q58F55, Q9NRT6,
UniProt Related Accession:	Q9NS91
Molecular Weight:	495
NCBI Full Name:	E3 ubiquitin-protein ligase RAD18
NCBI Synonym Full Names:	RAD18 E3 ubiquitin protein ligase
NCBI Official Symbol:	RAD18
NCBI Official Synonym Symbols:	RNF73
NCBI Protein Information:	E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18 homolog; RING finger protein 73; RAD18, S. cerevisiae, homolog; postreplication repair protein RAD18; postreplication repair protein hRAD18p
UniProt Protein Name:	E3 ubiquitin-protein ligase RAD18
UniProt Synonym Protein Names:	Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RAD18
UniProt Entry Name:	RAD18_HUMAN

	Western blot analysis of extracts of various cell lines, using Rad18 antibody (CAB13434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
	Immunofluorescence analysis of U2OS cells using Rad18 antibody (CAB13434). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using Rad18 antibody (CAB13434). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.