Cell Cycle Antibodies 1

Anti-RAD17 Antibody (CAB5359)

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SKU:
CAB5359
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-RAD17 Antibody
Antibody SKU:CAB5359
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:Human
Positive Samples:SW480, BT-474, K-562, 22Rv1, Raji
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KILY CKRA SLTE LDSP RLPS HLSE YERD TLLV EPEE VVEM SHMP GDLF NLYL HQNY IDFF MEID DIVR ASEF LSFA DILS GDWN TRSL LREY STSI ATRG VMHS NKAR GYAH CQGG GSSF RPLH KPQW FLIN KKYR ENCL AAKA LFPD FCLP ALCL QTQL LPYL ALLT IPMR NQAQ ISFI QDIG RLPL KRHF GRLK MEAL TDRE HGMI DPDS GDEA QLNG GHSA EESL GEPT QATV PETW SLPL SQNS ASEL PASQ PQPF SAQG DMEE NIII EDYE SDGT
Gene ID:5884
Uniprot:O75943
Cellular Location:Nucleus
Calculated MW:57kDa/66kDa/75kDa/77kDa
Observed MW:77kDa
Synonyms:RAD17, CCYC, HRAD17, R24L, RAD17SP, RAD24
Background:The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
UniProt Protein Function:RAD17: a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. Shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. Binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. Phosphorylated and activated after DNA damage, inducing cell cycle G2 arrest. Eight alternatively spliced variants have been reported.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm; chromosome, telomeric region; nucleus

Molecular Function:protein binding; ATP binding

Biological Process: regulation of phosphorylation; negative regulation of DNA replication; mitotic cell cycle checkpoint; DNA damage checkpoint; DNA repair; DNA replication; DNA replication checkpoint; response to DNA damage stimulus

NCBI Summary:The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]
UniProt Code:O75943
NCBI GenInfo Identifier:62287484
NCBI Gene ID:5884
NCBI Accession:O75943.2
UniProt Secondary Accession:O75943,O75714, Q7Z3S4, Q9UNK7, Q9UNR7, Q9UNR8, Q9UPF5 A8K8X2, D3DWA5,
UniProt Related Accession:O75943
Molecular Weight:681
NCBI Full Name:Cell cycle checkpoint protein RAD17
NCBI Synonym Full Names:RAD17 homolog (S. pombe)
NCBI Official Symbol:RAD17  
NCBI Official Synonym Symbols:CCYC; R24L; RAD24; HRAD17; RAD17SP  
NCBI Protein Information:cell cycle checkpoint protein RAD17; RAD1 homolog; Rad17-like protein; RF-C activator 1 homolog; RF-C/activator 1 homolog
UniProt Protein Name:Cell cycle checkpoint protein RAD17
UniProt Synonym Protein Names:RF-C/activator 1 homolog
Protein Family:Checkpoint protein
UniProt Gene Name:RAD17  
UniProt Entry Name:RAD17_HUMAN
Anti-RAD17 Antibody (CAB5359)Western blot analysis of extracts of various cell lines, using RAD17 antibody (CAB5359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-RAD17 Antibody (CAB5359)Immunofluorescence analysis of A549 cells using RAD17 antibody (CAB5359).
Anti-RAD17 Antibody (CAB5359)Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug RAD17 antibody (CAB5359). Western blot was performed from the immunoprecipitate using RAD17 antibody (CAB5359) at a dilition of 1:1000.
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