Autophagy Antibodies

Anti-RAB8A Antibody (CAB2810)

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SKU:
CAB2810
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-RAB8A Antibody (CAB2810)
Antibody SKU:CAB2810
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-207 of human RAB8A (NP_005361.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa, HCT116
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-207 of human RAB8A (NP_005361.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RNWI RNIE EHAS ADVE KMIL GNKC DVND KRQV SKER GEKL ALDY GIKF METS AKAN INVE NAFF TLAR DIKA KMDK KLEG NSPQ GSNQ GVKI TPDQ QKRS SFFR CVLL
Cellular Location:Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Lipid-anchor, Recycling endosome membrane, centriole, centrosome, cilium, cilium basal body, cytoskeleton, microtubule organizing center, phagosome, phagosome membrane
Calculated MW:23kDa
Observed MW:25kDa
Synonyms:RAB8A, MEL, RAB8
Background:The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
Western blot analysis of extracts of HCT116 cells, using RAB8A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of HCT116 cells, using RAB8A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Western blot analysis of extracts of HeLa cells, using RAB8A antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Western blot analysis of extracts of HeLa cells, using RAB8A antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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