Name: Anti-RAB5A Antibody (CAB1180)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB1180
Availability: OutOfStock

Antibody Name:	Anti-RAB5A Antibody [KO Validated]
Antibody SKU:	CAB1180
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5A (NP_004153.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, PC-12, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RAB5A (NP_004153.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASR GATR PNGP NTGN KICQ FKLV LLGE SAVG KSSL VLRF VKGQ FHEF QEST IGAA FLTQ TVCL DDTT VKFE IWDT AGQE RYHS LAPM YYRG AQAA IVVY DITN EESF ARAK NWVK ELQR QASP NIVI ALSG NKAD LANK RAVD FQEA QSYA DDNS LLFM ETSA KTSM NVNE IFMA IAKK LPKN EPQN PGAN SARG RGVD LTEP TQPT RNQC CSN
Gene ID:	5868
Uniprot:	P20339
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Early endosome membrane, Lipid-anchor, Melanosome, Membrane, cytosol, ruffle
Calculated MW:	22kDa/23kDa
Observed MW:	22kDa

Synonyms:	RAB5A, RAB5
Background:	RAB5A (RAB5A, member RAS oncogene family) is a protein-coding gene. Diseases associated with RAB5A include borna disease, and venezuelan equine encephalitis. GO annotations related to this gene include GDP binding and GTP binding. An important paralog of this gene is RAB20.

UniProt Protein Function:	RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:	Protein type:G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 3p24-p22 Cellular Component: zymogen granule membrane; protein complex; early endosome; dendrite; terminal button; phagocytic vesicle; cytosol; actin cytoskeleton; lipid raft; ruffle; synaptic vesicle; recycling endosome; cell soma; endocytic vesicle; perinuclear region of cytoplasm; axon; cytoplasm; melanosome; plasma membrane; endosome membrane; nerve terminal; endosome Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; blood coagulation; positive regulation of smooth muscle cell migration; Rab protein signal transduction; positive regulation of exocytosis
UniProt Code:	P20339
NCBI GenInfo Identifier:	1346958
NCBI Gene ID:	5868
NCBI Accession:	P20339.2
UniProt Secondary Accession:	P20339,Q6FI44, B4DJA5,
UniProt Related Accession:	P20339
Molecular Weight:	22,178 Da
NCBI Full Name:	Ras-related protein Rab-5A
NCBI Synonym Full Names:	RAB5A, member RAS oncogene family
NCBI Official Symbol:	RAB5A
NCBI Official Synonym Symbols:	RAB5
NCBI Protein Information:	ras-related protein Rab-5A; RAS-associated protein RAB5A
UniProt Protein Name:	Ras-related protein Rab-5A
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB5A
UniProt Entry Name:	RAB5A_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB5A antibody (CAB1180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Western blot analysis of extracts from normal (control) and RAB5A knockout (KO) HeLa cells, using RAB5A antibody (CAB1180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of C6 cells using [KO Validated] RAB5A Rabbit pAb (CAB1180) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] RAB5A Rabbit pAb (CAB1180) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using [KO Validated] RAB5A Rabbit pAb (CAB1180) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.