Anti-Rab4 Antibody

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SKU:
STPA00019
Antibody Type:
Polyclonal
Applications:
WB
Applications:
IHC
Applications:
ICC/IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019) at 1:150 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT.
  • Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Rab4 protein using Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
  • Immunohistochemistry analysis using Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Epidermis (cell-cell border and cytoplasmic), hair follicles and muscle.
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: String nuclear and cytoplasmic staining.
  • Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab4 Polyclonal Antibody (STPA00019) at 1:150 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT.
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Description

Rab4 Antibody

Rab4 Antibody is a rabbit polyclonal antibody directed against Rab4, offered by Assay Genie for research applications. Reported applications include WB, IHC and ICC/IF. Reported reactivity: Human, Mouse and Rat. Purified by Peptide Affinity.

Rab4 is a 25 kDa GTPase of the Ras superfamily that regulates early endosomal recycling. It cycles between an inactive GDP-bound state and an active GTP-bound state, recruiting effector proteins to intracellular membranes. Rab4 modulates receptor recycling, including transferrin and glucose transporter (GLUT4) trafficking. Overexpression of Rab4 alters receptor distribution, leading to accumulation in post-endosomal compartments. Its activity is regulated by phosphorylation and post-translational lipid modifications, which influence membrane association and subcellular localization. In neurons, Rab4 is involved in synaptic vesicle recycling and receptor trafficking. Dysregulation of Rab4 may impair endosomal sorting and contribute to synaptic dysfunction in neurodegenerative diseases such as Alzheimer’s and Parkinson’s. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µl. For research use only; not for diagnostic or therapeutic procedures.

Product Name:Rab4 Antibody
Product SKU:STPA00019
Size:100 µl
Target:Rab4
Host Species:Rabbit
Antibody Type:Polyclonal
Research Areas:Cell Signaling, Tags and Cell Markers, Organelle Markers, Neuroscience, Cell Markers, Neuron Markers, Presynaptic Markers
Buffer:PBS pH7.4, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, ICC/IF
Purification:Peptide Affinity Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse, Rat
Recommended Dilution:WB (1:1000), IHC (1:100), ICC/IF (1:150); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~26kDa.
Guarantee:12 months from date of dispatch
Immunogen:C-terminal peptide from human Rab4
Immunogen Species:Human
Accession Number:NP_004569.2
Gene ID:5867
Swiss-Prot:P20338
Cellular Localization:Membrane | Cytoplasm
Alternative Names:Oncogene Rab4, Rab4A, Ras related protein
References:1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886. 6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507. 7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11(12), 4379-4389.
Note:For research use only.
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