Name: Anti-RAB27A Antibody (CAB1934)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB1934
Availability: OutOfStock

Antibody Name:	Anti-RAB27A Antibody [KO Validated]
Antibody SKU:	CAB1934
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-937

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSDG DYDY LIKF LALG DSGV GKTS VLYQ YTDG KFNS KFIT TVGI DFRE KRVV YRAS GPDG ATGR GQRI HLQL WDTA GQER FRSL TTAF FRDA MGFL LLFD LTNE QSFL NVRN WISQ LQMH AYCE NPDI VLCG NKSD LEDQ RVVK EEEA IALA EKYG IPYF ETSA ANGT NISQ AIEM LLDL IMKR MERC VDKS WIPE GVVR SNGH ASTD QLSE EKEK GACG C
Gene ID:	5873
Uniprot:	P51159
Cellular Location:	Late endosome, Lipid-anchor, Lysosome, Melanosome, Membrane
Calculated MW:	24kDa
Observed MW:	25kDa

Synonyms:	RAB27A, GS2, HsT18676, RAB27, RAM
Background:	The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

UniProt Protein Function:	RAB27A: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2). Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:G protein, monomeric; G protein, monomeric, Rab Chromosomal Location of Human Ortholog: 15q15-q21.1 Cellular Component: Golgi apparatus; photoreceptor outer segment; lysosome; secretory granule membrane; apical plasma membrane; dendrite; late endosome; melanosome; secretory granule Molecular Function:GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding Biological Process: exocytosis; natural killer cell degranulation; cytotoxic T cell degranulation; melanosome localization; protein secretion; synaptic vesicle transport; melanosome transport; antigen processing and presentation; intracellular protein transport; cellular protein metabolic process; positive regulation of phagocytosis; melanocyte differentiation; Rab protein signal transduction; blood coagulation; vesicle docking during exocytosis; protein targeting; positive regulation of exocytosis Disease: Griscelli Syndrome, Type 2
NCBI Summary:	The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P51159
NCBI GenInfo Identifier:	116242744
NCBI Gene ID:	5873
NCBI Accession:	P51159.3
UniProt Related Accession:	P51159
Molecular Weight:
NCBI Full Name:	Ras-related protein Rab-27A
NCBI Synonym Full Names:	RAB27A, member RAS oncogene family
NCBI Official Symbol:	RAB27A
NCBI Official Synonym Symbols:	GS2; RAM; RAB27; HsT18676
NCBI Protein Information:	ras-related protein Rab-27A
UniProt Protein Name:	Ras-related protein Rab-27A
UniProt Synonym Protein Names:	GTP-binding protein Ram
UniProt Gene Name:	RAB27A
UniProt Entry Name:	RB27A_HUMAN

	Western blot analysis of extracts of U-937 cells, using RAB27A antibody (CAB1934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Western blot analysis of extracts from normal (control) and RAB27A knockout (KO) HeLa cells, using RAB27A antibody (CAB1934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human adenomyosis using RAB27A Antibody (CAB1934) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using RAB27A Antibody (CAB1934) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of L929 cells using RAB27A antibody (CAB1934) at dilution of 1:100. Blue: DAPI for nuclear staining.