Autophagy Antibodies
Anti-RAB1B Antibody (CAB7514)
- SKU:
- CAB7514
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-RAB1B Antibody |
| Antibody SKU: | CAB7514 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, SKOV3, 293T, MCF7, Mouse kidney, Mouse heart, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YLFK LLLI GDSG VGKS CLLL RFAD DTYT ESYI STIG VDFK IRTI ELDG KTIK LQIW DTAG QERF RTIT SSYY RGAH GIIV VYDV TDQE SYAN VKQW LQEI DRYA SENV NKLL VGNK SDLT TKKV VDNT TAKE FADS LGIP FLET SAKN ATNV EQAF MTMA AEIK KRMG PGAA SGGE RPNL KIDS TPVK PAGG GCC |
| Gene ID: | 81876 |
| Uniprot: | Q9H0U4 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Lipid-anchor, Membrane, Preautophagosomal structure membrane |
| Calculated MW: | 22kDa |
| Observed MW: | 28kDa |
| Synonyms: | RAB1B |
| Background: | Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009] |
| UniProt Protein Function: | RAB1B: Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Belongs to the small GTPase superfamily. Rab family. |
| UniProt Protein Details: | Protein type:G protein, monomeric; G protein, monomeric, Rab; G protein Chromosomal Location of Human Ortholog: 11q12 Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; pre-autophagosomal structure membrane; ER-Golgi intermediate compartment Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; autophagy; virus assembly; Rab protein signal transduction |
| NCBI Summary: | Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi (Chen et al., 1997 [PubMed 9030196]; Alvarez et al., 2003 [PubMed 12802079]).[supplied by OMIM, Jan 2009] |
| UniProt Code: | Q9H0U4 |
| NCBI GenInfo Identifier: | 23396834 |
| NCBI Gene ID: | 81876 |
| NCBI Accession: | Q9H0U4.1 |
| UniProt Secondary Accession: | Q9H0U4,A8K7S1, |
| UniProt Related Accession: | Q9H0U4 |
| Molecular Weight: | 201 |
| NCBI Full Name: | Ras-related protein Rab-1B |
| NCBI Synonym Full Names: | RAB1B, member RAS oncogene family |
| NCBI Official Symbol: | RAB1B |
| NCBI Protein Information: | ras-related protein Rab-1B; small GTP-binding protein |
| UniProt Protein Name: | Ras-related protein Rab-1B |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB1B |
| UniProt Entry Name: | RAB1B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using RAB1B antibody (CAB7514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human kidney using RAB1B antibody (CAB7514) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using RAB1B Rabbit pAb (CAB7514) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using RAB1B Rabbit pAb (CAB7514) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using RAB1B Rabbit pAb (CAB7514) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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