Name: Anti-PTPRF Antibody (CAB5444)
Brand: Cell Biology Antibodies 9
SKU: CAB5444
Availability: OutOfStock

Antibody Name:	Anti-PTPRF Antibody
Antibody SKU:	CAB5444
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human PTPRF (NP_569707.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	MCF7, THP-1, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-190 of human PTPRF (NP_569707.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DSKP VFIK VPED QTGL SGGV ASFV CQAT GEPK PRIT WMKK GKKV SSQR FEVI EFDD GAGS VLRI QPLR VQRD EAIY ECTA TNSL GEIN TSAK LSVL EEEQ LPPG FPSI DMGP QLKV VEKA RTAT MLCA AGGN PDPE ISWF KDFL PVDP ATSN GRIK QLRS G
Gene ID:	5792
Uniprot:	P10586
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	211kDa/212kDa
Observed MW:	150kDa

Synonyms:

PTPRF, BNAH2, LAR

Background:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

UniProt Protein Function:	PTPRF: Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase). Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.1.3.48; Receptor protein phosphatase, tyrosine Chromosomal Location of Human Ortholog: 1p34 Cellular Component: integral to plasma membrane Molecular Function:heparin binding; transmembrane receptor protein tyrosine phosphatase activity; protein complex binding; protein tyrosine phosphatase activity Biological Process: cell migration; cell adhesion; transmembrane receptor protein tyrosine phosphatase signaling pathway Disease: Breasts And/or Nipples, Aplasia Or Hypoplasia Of, 2
NCBI Summary:	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P10586
NCBI GenInfo Identifier:	226709091
NCBI Gene ID:	5792
NCBI Accession:	P10586.2
UniProt Secondary Accession:	P10586,Q5T021, Q5T022, Q5W9G2, Q86WS0, D3DPX6, D3DPX7
UniProt Related Accession:	P10586
Molecular Weight:	1907
NCBI Full Name:	Receptor-type tyrosine-protein phosphatase F
NCBI Synonym Full Names:	protein tyrosine phosphatase, receptor type, F
NCBI Official Symbol:	PTPRF
NCBI Official Synonym Symbols:	LAR; BNAH2
NCBI Protein Information:	receptor-type tyrosine-protein phosphatase F; LCA-homolog; leukocyte common antigen related; leukocyte antigen-related (LAR) PTP receptor; leukocyte antigen-related tyrosine phosphatase; receptor-linked protein-tyrosine phosphatase LAR; protein tyrosine phosphatase, receptor type, F polypeptide
UniProt Protein Name:	Receptor-type tyrosine-protein phosphatase F
UniProt Synonym Protein Names:	Leukocyte common antigen related; LAR
Protein Family:	Receptor-type tyrosine-protein phosphatase
UniProt Gene Name:	PTPRF
UniProt Entry Name:	PTPRF_HUMAN

Western blot analysis of extracts of various cell lines, using PTPRF antibody (CAB5444) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

ELISA Kits

Human PTPRF / Receptor-type tyrosine-protein phosphatase F ELISA Kit

Human Receptor-type tyrosine-protein phosphatase F (PTPRF) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)