Antibody Name:	Anti-PSMC6 Antibody
Antibody SKU:	CAB5377
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human PSMC6 (NP_002797.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	NCI-H460, 293T, SH-SY5Y, Mouse liver, Mouse kidney, Mouse brain, Mouse heart, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human PSMC6 (NP_002797.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DKAL QDYR KKLL EHKE IDGR LKEL REQL KELT KQYE KSEN DLKA LQSV GQIV GEVL KQLT EEKF IVKA TNGP RYVV GCRR QLDK SKLK PGTR VALD MTTL TIMR YLPR EVDP LVYN MSHE DPGN VSYS EIG
Gene ID:	5706
Uniprot:	P62333
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	44kDa
Observed MW:	45kDa

Synonyms:

PSMC6, CADP44, HEL-S-73, P44, SUG2, p42, ATPase 6

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12.

UniProt Protein Function:	RPT4: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family.
UniProt Protein Details:	Protein type:Proteasome complex; Protease Chromosomal Location of Human Ortholog: 14q22.1 Cellular Component: nucleoplasm; proteasome complex; membrane; nucleus; cytosol Molecular Function:protein binding, bridging; protein binding; ATPase activity; ATP binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008]
UniProt Code:	P62333
NCBI GenInfo Identifier:	51702772
NCBI Gene ID:	5706
NCBI Accession:	P62333.1
UniProt Secondary Accession:	P62333,P49719, Q6IBU3, Q92524, B2R975,
UniProt Related Accession:	P62333
Molecular Weight:	389
NCBI Full Name:	26S protease regulatory subunit 10B
NCBI Synonym Full Names:	proteasome (prosome, macropain) 26S subunit, ATPase, 6
NCBI Official Symbol:	PSMC6
NCBI Official Synonym Symbols:	P44; p42; SUG2; CADP44; HEL-S-73
NCBI Protein Information:	26S protease regulatory subunit 10B; proteasome subunit p42; proteasome 26S subunit ATPase 6; conserved ATPase domain protein 44; epididymis secretory protein Li 73; 26S protease regulatory subunit S10B; 26S proteasome AAA-ATPase subunit RPT4
UniProt Protein Name:	26S protease regulatory subunit 10B
UniProt Synonym Protein Names:	26S proteasome AAA-ATPase subunit RPT4; Proteasome 26S subunit ATPase 6; Proteasome subunit p42
Protein Family:	26S protease regulatory
UniProt Gene Name:	PSMC6
UniProt Entry Name:	PRS10_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMC6 antibody (CAB5377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney using PSMC6 antibody (CAB5377) at dilution of 1:100 (40x lens).