Antibody Name:	Anti-PSMC2 Antibody
Antibody SKU:	CAB1985
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PSMC2 (NP_002794.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, SW620, BT474, DUCAR3, 293T, mouse spleen, mouse brain, rat testis, rat liver, BT-474, OVCAR3, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PSMC2 (NP_002794.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPDY LGAD QRKT KEDE KDDK PIRA LDEG DIAL LKTY GQST YSRQ IKQV EDDI QQLL KKIN ELTG IKES DTGL APPA LWDL AADK QTLQ SEQP LQVA RCTK IINA DSED PKYI INVK QFAK FVVD LSDQ VAPT DIEE GMRV GVDR NKYQ IHIP LPPK IDPT
Gene ID:	5701
Uniprot:	P35998
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	33kDa/48kDa
Observed MW:	49kDa

Synonyms:

PSMC2, MSS1, Nbla10058, S7, ATPase 2

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	RPT1: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, positive modulator of Tat-mediated transactivation. Belongs to the AAA ATPase family.
UniProt Protein Details:	Protein type:Proteasome complex; Protease Chromosomal Location of Human Ortholog: 7q22.1-q22.3 Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; membrane; nucleoplasm; nucleus; proteasome complex Molecular Function:ATPase activity; protein binding; TATA-binding protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; ER-associated protein catabolic process; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; osteoblast differentiation; positive regulation of transcriptional preinitiation complex assembly; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2011]
UniProt Code:	P35998
NCBI GenInfo Identifier:	547930
NCBI Gene ID:	5701
NCBI Accession:	P35998.3
UniProt Secondary Accession:	P35998,Q3LIA5, Q9UDI3, A4D0Q1, B7Z5E2,
UniProt Related Accession:	P35998
Molecular Weight:	33,274 Da
NCBI Full Name:	26S protease regulatory subunit 7
NCBI Synonym Full Names:	proteasome 26S subunit, ATPase 2
NCBI Official Symbol:	PSMC2
NCBI Official Synonym Symbols:	S7; MSS1; Nbla10058
NCBI Protein Information:	26S protease regulatory subunit 7
UniProt Protein Name:	26S protease regulatory subunit 7
UniProt Synonym Protein Names:	26S proteasome AAA-ATPase subunit RPT1; Proteasome 26S subunit ATPase 2; Protein MSS1
UniProt Gene Name:	PSMC2
UniProt Entry Name:	PRS7_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMC2 antibody (CAB1985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded mouse heart using PSMC2 antibody (CAB1985) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PSMC2 antibody (CAB1985) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PSMC2 antibody (CAB1985). Blue: DAPI for nuclear staining.