Developmental Biology

Anti-Protein Jumonji Antibody (CAB9823)

(No reviews yet) Write a Review
SKU:
CAB9823
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Protein Jumonji Antibody
Antibody SKU:CAB9823
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1027-1246 of human JARID2 (NP_004964.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, Mouse thymus, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1027-1246 of human JARID2 (NP_004964.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ETVH FATT QWTS MGFE TAKE MKRR HIAK PFSM EKLL YQIA QAEA KKEN GPTL STIS ALLD ELRD TELR QRRQ LFEA GLHS SARY GSHD GSST VADG KKKP RKWL QLET SERR CQIC QHLC YLSM VVQE NENV VFCL ECAL RHVE KQKS CRGL KLMY RYDE EQII SLVN QICG KVSG KNGS IENC LSKP TPKR GPRK RATV DVPP SRLS ASSS SKSA SSSS
Gene ID:3720
Uniprot:Q92833
Cellular Location:Nucleus
Calculated MW:106kDa/119kDa/138kDa
Observed MW:160kDa
Synonyms:JARID2, JMJ
Background:This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies.
UniProt Protein Function:JARID2: a Polycomb protein required for neural tube formation. Contains a Jumonji C domain but lacks histone demethylase activity. Essential for normal heart development and function. Required for embryonic viability. Down-regulation of Jarid2 impairs ES cell differentiation and Oct4 silencing. Associates with the Polycomb-Repressive Complex 2 (PRC2), stimulating PRC2 methyltransferase activity. PRC2 includes the histone H3 Lys 27 (H3K27) methyltransferase Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells. The ability of Jarid 2 to be recruited to DNA in ES cells is interdependent on PRC2. Its knockdown reduces PRC2 at its target promoters, while ES cells lacking the PRC2 component EED are deficient in Jarid2 promoter activity. Acts as a transcriptional repressor of ANF by binding to both GATA4 and NKX2-5 and repressing their transcriptional activator activities. Participates in the negative regulation of cell proliferation signaling. Interacts with the N-terminal region of GATA4 and the C- terminal region of the NKX2-5 homeobox and represses their ability to activate transcription of ANF.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6p24-p23

Cellular Component: ESC/E(Z) complex; histone methyltransferase complex; nucleoplasm; nucleus

Molecular Function:chromatin binding; histone demethylase activity

Biological Process: central nervous system development; chromatin modification; liver development; negative regulation of cell proliferation; negative regulation of gene expression, epigenetic; negative regulation of histone methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K9 methylation; spleen development; stem cell differentiation; thymus development; transcription, DNA-dependent

NCBI Summary:This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012]
UniProt Code:Q92833
NCBI GenInfo Identifier:11863152
NCBI Gene ID:3720
NCBI Accession:NP_004964.2
UniProt Secondary Accession:Q92833,Q5U5L5, Q86X63, A8K9Z6, B7Z5S5, B7Z8L0,
UniProt Related Accession:Q92833
Molecular Weight:119,689 Da
NCBI Full Name:protein Jumonji isoform 1
NCBI Synonym Full Names:jumonji and AT-rich interaction domain containing 2
NCBI Official Symbol:JARID2  
NCBI Official Synonym Symbols:JMJ  
NCBI Protein Information:protein Jumonji
UniProt Protein Name:Protein Jumonji
UniProt Synonym Protein Names:Jumonji/ARID domain-containing protein 2
UniProt Gene Name:JARID2  
UniProt Entry Name:JARD2_HUMAN
Anti-Protein Jumonji Polyclonal Antibody (CAB9823)Western blot analysis of extracts of various cell lines, using JARID2 antibody (CAB9823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
View AllClose

0 Reviews

View AllClose

Related Products