Name: Anti-PRMT8 Antibody (CAB18440)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB18440
Availability: OutOfStock

Antibody Name:	Anti-PRMT8 Antibody [KO Validated]
Antibody SKU:	CAB18440
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human PRMT8.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Mouse brain, Rat brain

Immunogen:	Recombinant protein of human PRMT8.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	56341
Uniprot:	Q9NR22
Cellular Location:	Cell membrane, Cytoplasmic side, Lipid-anchor
Calculated MW:	44kDa/45kDa
Observed MW:	42KDa

	Western blot analysis of extracts of various cell lines, using PRMT8 antibody (CAB18440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and PRMT8 Rabbit pAb knockout (KO) 293T cells, using PRMT8 Rabbit pAb antibody (CAB18440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:	HRMT1L3, HRMT1L4, PRMT8
Background:	Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]

UniProt Protein Function:	PRMT8: Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS. Belongs to the protein arginine N-methyltransferase family. PRMT8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase, protein arginine; EC 2.1.1.-; Methyltransferase Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: cytosol; nucleus; plasma membrane Molecular Function:histone-arginine N-methyltransferase activity; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity Biological Process: histone methylation; peptidyl-arginine methylation; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; regulation of protein binding; regulation of transcription, DNA-dependent
NCBI Summary:	Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9NR22
NCBI GenInfo Identifier:	88983969
NCBI Gene ID:	56341
NCBI Accession:	Q9NR22.2
UniProt Secondary Accession:	Q9NR22,Q8TBJ8, B2RDP0,
UniProt Related Accession:	Q9NR22
Molecular Weight:	44,169 Da
NCBI Full Name:	Protein arginine N-methyltransferase 8
NCBI Synonym Full Names:	protein arginine methyltransferase 8
NCBI Official Symbol:	PRMT8
NCBI Official Synonym Symbols:	HRMT1L3; HRMT1L4
NCBI Protein Information:	protein arginine N-methyltransferase 8
UniProt Protein Name:	Protein arginine N-methyltransferase 8
UniProt Synonym Protein Names:	Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
Protein Family:	Protein arginine N-methyltransferase
UniProt Gene Name:	PRMT8
UniProt Entry Name:	ANM8_HUMAN