Signal Transduction Antibodies 2
Anti-PRG4 Antibody (CAB15379)
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- SKU:
- CAB15379
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-PRG4 Antibody |
| Antibody SKU: | CAB15379 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ERAI GPSQ THTI RIQY SPAR LAYQ DKGV LHNE VKVS ILWR GLPN VVTS AISL PNIR KPDG YDYY AFSK DQYY NIDV PSRT ARAI TTRS GQTL SKVW YNCP |
| Gene ID: | 10216 |
| Uniprot: | Q92954 |
| Cellular Location: | Secreted |
| Calculated MW: | 102kDa/136kDa/141kDa/146kDa/151kDa |
| Observed MW: | 150kDa |
| Synonyms: | PRG4, CACP, HAPO, JCAP, MSF, SZP |
| Background: | The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1q25-q31 Cellular Component: extracellular space Molecular Function:scavenger receptor activity; polysaccharide binding Biological Process: receptor-mediated endocytosis; cell proliferation; negative regulation of interleukin-6 biosynthetic process; immune response; regulation of cell proliferation Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome |
| NCBI Summary: | The protein encoded by this gene is a large proteoglycan specifically synthesized by chondrocytes located at the surface of articular cartilage, and also by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92954 |
| NCBI GenInfo Identifier: | 83288393 |
| NCBI Gene ID: | 10216 |
| NCBI Accession: | Q92954.2 |
| UniProt Secondary Accession: | Q92954,Q6DNC4, Q6DNC5, Q6ZMZ5, Q9BX49, |
| UniProt Related Accession: | Q92954 |
| Molecular Weight: | 151,077 Da |
| NCBI Full Name: | Proteoglycan 4 |
| NCBI Synonym Full Names: | proteoglycan 4 |
| NCBI Official Symbol: | PRG4 |
| NCBI Official Synonym Symbols: | MSF; SZP; CACP; HAPO; JCAP; bG174L6.2 |
| NCBI Protein Information: | proteoglycan 4; lubricin; superficial zone proteoglycan; megakaryocyte stimulating factor; megakaryocyte-stimulating factor; articular superficial zone protein; bG174L6.2 (MSF: megakaryocyte stimulating factor ) |
| UniProt Protein Name: | Proteoglycan 4 |
| UniProt Synonym Protein Names: | Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycan |
| Protein Family: | Proteoglycan |
| UniProt Gene Name: | PRG4 |
| UniProt Entry Name: | PRG4_HUMAN |
 | Western blot analysis of extracts of LO2 cells, using PRG4 antibody (CAB15379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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