Anti-PREB Antibody (CAB20957)
- SKU:
- CAB20957
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-PREB Antibody (CAB20957) |
| Antibody SKU: | CAB20957 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human PREB. |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, MCF7, K-562, Mouse brain, Rat brain |
| Immunogen: | Recombinant protein of Human PREB. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Endoplasmic reticulum membrane, Nucleus, Single-pass membrane protein |
| Calculated MW: | 45kDa |
| Observed MW: | 46KDa |
| Synonyms: | PREB, SEC12 |
| Background: | This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand. |
 | Immunofluorescence analysis of PC-12 cells using PREB Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using PREB antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
 | Immunofluorescence analysis of NIH/3T3 cells using PREB Rabbit mAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using PREB antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
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