KO Validated Antibodies 1

Anti-PRDX4 Antibody (CAB1486)[KO Validated]

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SKU:
CAB1486
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PRDX4 Antibody [KO Validated]
Antibody SKU:CAB1486
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human PRDX4 (NP_006397.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:H460, PC-3, HeLa, MCF-7, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human PRDX4 (NP_006397.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WETE ERPR TREE ECHF YAGG QVYP GEAS RVSV ADHS LHLS KAKI SKPA PYWE GTAV IDGE FKEL KLTD YRGK YLVF FFYP LDFT FVCP TEII AFGD RLEE FRSI NTEV VACS VDSQ FTHL AWIN TPRR QGGL GPIR IPLL SDLT HQIS KDYG VYLE DSGH TLRG LFII DDKG ILRQ ITLN DLPV GRSV DETL RLVQ AFQY TDKH GEVC PAGW KPGS ETII PDPA GKLK YFDK LN
Gene ID:10549
Uniprot:Q13162
Cellular Location:Cytoplasm, Secreted
Calculated MW:30kDa
Observed MW:28kDa
Synonyms:PRDX4, AOE37-2, AOE372, HEL-S-97n, PRX-4
Background: The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
UniProt Protein Function:PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.11.1.15; Secreted; Mitochondrial; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: cytosol; extracellular space; mitochondrion; nucleus; smooth endoplasmic reticulum

Molecular Function:protein binding; protein homodimerization activity; thioredoxin peroxidase activity

Biological Process: 4-hydroxyproline metabolic process; cell redox homeostasis; extracellular matrix organization and biogenesis; I-kappaB phosphorylation; male gonad development; protein maturation via protein folding; spermatogenesis

NCBI Summary:The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]
UniProt Code:Q13162
NCBI GenInfo Identifier:3024727
NCBI Gene ID:10549
NCBI Accession:Q13162.1
UniProt Secondary Accession:Q13162,Q6FHT3,
UniProt Related Accession:Q13162
Molecular Weight:
NCBI Full Name:Peroxiredoxin-4
NCBI Synonym Full Names:peroxiredoxin 4
NCBI Official Symbol:PRDX4  
NCBI Official Synonym Symbols:PRX-4; AOE372; AOE37-2; HEL-S-97n  
NCBI Protein Information:peroxiredoxin-4
UniProt Protein Name:Peroxiredoxin-4
UniProt Synonym Protein Names:Antioxidant enzyme AOE372; AOE37-2; Peroxiredoxin IV; Prx-IV; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372
Protein Family:Peroxiredoxin
UniProt Gene Name:PRDX4  
UniProt Entry Name:PRDX4_HUMAN
Anti-PRDX4 Antibody [KO Validated] (CAB1486)Western blot analysis of extracts of various cell lines, using PRDX4 antibody (CAB1486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PRDX4 Antibody [KO Validated] (CAB1486)Western blot analysis of extracts from normal (control) and PRDX4 knockout (KO) 293T cells, using PRDX4 antibody (CAB1486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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