Cell Biology Antibodies 8
Anti-PPP4C Antibody (CAB2109)
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- SKU:
- CAB2109
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PPP4C Antibody |
| Antibody SKU: | CAB2109 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, NIH/3T3, MCF-7, HO-8910PM, Mouse testis, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEI SDLD RQIE QLRR CELI KESE VKAL CAKA REIL VEES NVQR VDSP VTVC GDIH GQFY DLKE LFRV GGDV PETN YLFM GDFV DRGF YSVE TFLL LLAL KVRY PDRI TLIR GNHE SRQI TQVY GFYD ECLR KYGS VTVW RYCT EIFD YLSL SAII DGKI FCVH GGLS PSIQ TLDQ IRTI DRKQ EVPH DGPM CDLL WSDP EDTT GWGV SPRG AGYL FGSD VVAQ FNAA NDID MICR AHQL VMEG YKWH FNET VLTV WSAP NYCY RCGN VAAI LELD EHLQ KDFI IFEA APQE TRGI PSKK PVAD YFL |
| Gene ID: | 5531 |
| Uniprot: | P60510 |
| Cellular Location: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 35kDa |
| Observed MW: | 36kDa |
| Synonyms: | PPP4C, PP-X, PP4, PP4C, PPH3, PPP4, PPX |
| Background: |
| UniProt Protein Function: | PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1. |
| UniProt Protein Details: | Protein type:EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; microtubule organizing center; nucleus Molecular Function:NF-kappaB-inducing kinase activity; protein binding; metal ion binding; protein serine/threonine phosphatase activity Biological Process: dephosphorylation |
| UniProt Code: | P60510 |
| NCBI GenInfo Identifier: | 44888846 |
| NCBI Gene ID: | 5531 |
| NCBI Accession: | P60510.1 |
| UniProt Secondary Accession: | P60510,P33172, |
| UniProt Related Accession: | P60510 |
| Molecular Weight: | 307 |
| NCBI Full Name: | Serine/threonine-protein phosphatase 4 catalytic subunit |
| NCBI Synonym Full Names: | protein phosphatase 4, catalytic subunit |
| NCBI Official Symbol: | PPP4C |
| NCBI Official Synonym Symbols: | PP4; PPX; PP-X; PP4C; PPH3; PPP4 |
| NCBI Protein Information: | serine/threonine-protein phosphatase 4 catalytic subunit; protein phosphatase X, catalytic subunit; protein phosphatase 4 (formerly X), catalytic subunit |
| UniProt Protein Name: | Serine/threonine-protein phosphatase 4 catalytic subunit |
| UniProt Synonym Protein Names: | Protein phosphatase X; PP-X |
| UniProt Gene Name: | PPP4C |
| UniProt Entry Name: | PP4C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PPP4C antibody (CAB2109) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat liver using PPP4C antibody (CAB2109) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody (CAB2109) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using PPP4C antibody (CAB2109) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF-7 cells using PPP4C antibody (CAB2109). Blue: DAPI for nuclear staining. |
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