Cell Biology Antibodies 4

Anti-PPP4C Antibody (CAB13531)

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SKU:
CAB13531
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PPP4C Antibody
Antibody SKU:CAB13531
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, NIH3T3, MCF-7, Mouse testis, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human PPP4C (NP_002711.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAEI SDLD RQIE QLRR CELI KESE VKAL CAKA REIL VEES NVQR VDSP VTVC GDIH GQFY DLKE LFRV GGDV PETN YLFM GDFV DRGF YSVE TFLL LLAL KVRY PDRI TLIR GNHE SRQI TQVY GFYD ECLR KYGS VTVW RYCT EIFD YLSL SAII DGKI FCVH GGLS PSIQ TLDQ IRTI DRKQ EVPH DGPM CDLL WSDP EDTT GWGV SPRG AGYL FGSD VVAQ FNAA NDID MICR AHQL VMEG YKWH FNET VLTV WSAP NYCY RCGN VAAI LELD EHLQ KDFI IFEA APQE TRGI PSKK PVAD YFL
Gene ID:5531
Uniprot:P60510
Cellular Location:Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:35kDa
Observed MW:36kDa
Synonyms:PPP4C, PP-X, PP4, PP4C, PPH3, PPP4, PPX
Background:
UniProt Protein Function:PPP4C: a Ser/Thr phosphatase. A positive regulator of HPK1 and the HPK1-JNK signaling pathway. Mediates TNF-alpha-induced degradation of IRS-4 and plays a role in NF-kappaB p65 Thr dephosphorylation. Holoenzyme consists at least of a catalytic subunit PPP4C and a regulatory subunit PPP4R1.
UniProt Protein Details:

Protein type:EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; microtubule organizing center; nucleus

Molecular Function:NF-kappaB-inducing kinase activity; protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: dephosphorylation

UniProt Code:P60510
NCBI GenInfo Identifier:44888846
NCBI Gene ID:5531
NCBI Accession:P60510.1
UniProt Secondary Accession:P60510,P33172,
UniProt Related Accession:P60510
Molecular Weight:307
NCBI Full Name:Serine/threonine-protein phosphatase 4 catalytic subunit
NCBI Synonym Full Names:protein phosphatase 4, catalytic subunit
NCBI Official Symbol:PPP4C  
NCBI Official Synonym Symbols:PP4; PPX; PP-X; PP4C; PPH3; PPP4  
NCBI Protein Information:serine/threonine-protein phosphatase 4 catalytic subunit; protein phosphatase X, catalytic subunit; protein phosphatase 4 (formerly X), catalytic subunit
UniProt Protein Name:Serine/threonine-protein phosphatase 4 catalytic subunit
UniProt Synonym Protein Names:Protein phosphatase X; PP-X
UniProt Gene Name:PPP4C  
UniProt Entry Name:PP4C_HUMAN
Anti-PPP4C Antibody (CAB13531)Western blot analysis of extracts of various cell lines, using PPP4C antibody (CAB13531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-PPP4C Antibody (CAB13531)Immunofluorescence analysis of U2OS cells using PPP4C antibody (CAB13531). Blue: DAPI for nuclear staining.
Anti-PPP4C Antibody (CAB13531)Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug PPP4C antibody (CAB13531). Western blot was performed from the immunoprecipitate using PPP4C antibody (CAB13531) at a dilition of 1:1000.
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