Cell Biology Antibodies 4

Anti-PPP2R5B Antibody (CAB14252)

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SKU:
CAB14252
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PPP2R5B Antibody
Antibody SKU:CAB14252
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPP2R5B (NP_006235.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-87MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPP2R5B (NP_006235.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:METK LPPA STPT SPSS PGLS PVPP PDKV DGFS RRSL RRAR PRRS HSSS QFRY QSNQ QELT PLPL LKDV PASE LHEL LSRK LAQC GVMF DFLD CVAD LKGK EVKR AALN ELVE CVGS TRGV LIEP VYPD II
Gene ID:5526
Uniprot:Q15173
Cellular Location:Cytoplasm
Calculated MW:57kDa
Observed MW:57kDa
Synonyms:PPP2R5B, B56B, PR61B, B56beta
Background:The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily.
UniProt Protein Function:PPP2R5B: a brain-specific, B regulatory subunit of the PP2A phosphoprotein phosphatase that modulates substrate selectivity, localization, and catalytic activity of the holoenzyme. Targeted by the KLHL15-Cul3 ubiquitin ligase complex, promoting its turnover by ubiquitylation and proteasomal degradation. Mediates dephosphorylation of tyrosine hydroxylase (TH) and calcium/calmodulin-dependent protein kinase type II (CaMK2). The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 pT308 and pS373. Belongs to the phosphatase 2A regulatory subunit B56 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytoplasm; cytosol

Molecular Function:protein binding

Biological Process: regulation of phosphoinositide 3-kinase cascade

NCBI Summary:The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. [provided by RefSeq, Jul 2008]
UniProt Code:Q15173
NCBI GenInfo Identifier:7387497
NCBI Gene ID:5526
NCBI Accession:Q15173.1
UniProt Secondary Accession:Q15173,Q13853,
UniProt Related Accession:Q15173
Molecular Weight:57,294 Da
NCBI Full Name:Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
NCBI Synonym Full Names:protein phosphatase 2 regulatory subunit B'beta
NCBI Official Symbol:PPP2R5B  
NCBI Official Synonym Symbols:B56B; PR61B  
NCBI Protein Information:serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
UniProt Protein Name:Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
UniProt Synonym Protein Names:PP2A B subunit isoform B'-beta; PP2A B subunit isoform B56-beta; PP2A B subunit isoform PR61-beta; PP2A B subunit isoform R5-beta
Protein Family:Serine/threonine-protein phosphatase
UniProt Gene Name:PPP2R5B  
UniProt Entry Name:2A5B_HUMAN
Anti-PPP2R5B Antibody (CAB14252)Western blot analysis of extracts of U-87MG cells, using PPP2R5B antibody (CAB14252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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