Antibody Name:	Anti-PPP2R2A Antibody
Antibody SKU:	CAB2185
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PPP2R2A (NP_002708.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PPP2R2A (NP_002708.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGA GGGN DIQW CFSQ VKGA VDDD VAEA DIIS TVEF NHSG ELLA TGDK GGRV VIFQ QEQE NKIQ SHSR GEYN VYST FQSH EPEF DYLK SLEI EEKI NKIR WLPQ KNAA QFLL STND KTIK LWKI SERD KRPE GYNL KEED GRYR DPTT VTTL RVPV FRPM DLMV EASP RRIF ANAH TYHI NSIS INSD YETY LSAD DLRI NLWH LEIT DRSF NIVD IKPA NMEE LTEV ITAA EFHP NSCN TFVY SSSK GTIR LCDM RASA LCDR HSKL FEEP EDPS NRSF FSEI ISSI SDVK FSHS GRYM MTRD YLSV KIWD LNME NRPV ETYQ VHEY LRSK LCSL YEND CIFD KFEC CWNG SDSV VMTG SYNN FFRM FDRN TKRD ITLE ASRE NNKP RTVL KPRK VCAS GKRK KDEI SVDS LDFN KKIL HTAW HPKE NIIA VATT NNLY IFQD KVN
Gene ID:	5520
Uniprot:	P63151
Cellular Location:
Calculated MW:	51kDa/53kDa
Observed MW:	52kDa

Synonyms:

PPP2R2A, B55A, B55ALPHA, PR52A, PR55A, PR55alpha

Background:

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	PPP2R2A: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with TP53. Expressed in all tissues examined. Belongs to the phosphatase 2A regulatory subunit B family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 8p21.2 Cellular Component: cytosol; nucleoplasm; protein phosphatase type 2A complex Molecular Function:protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity Biological Process: G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation
NCBI Summary:	The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
UniProt Code:	P63151
NCBI GenInfo Identifier:	52783535
NCBI Gene ID:	5520
NCBI Accession:	P63151.1
UniProt Secondary Accession:	P63151,P50409, Q00007, B2RBU8, B4E1T7,
UniProt Related Accession:	P63151
Molecular Weight:	53,000 Da
NCBI Full Name:	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
NCBI Synonym Full Names:	protein phosphatase 2 regulatory subunit Balpha
NCBI Official Symbol:	PPP2R2A
NCBI Official Synonym Symbols:	B55A; PR52A; PR55A; B55ALPHA
NCBI Protein Information:	serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
UniProt Protein Name:	Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
UniProt Synonym Protein Names:	PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha
Protein Family:	Serine/threonine-protein phosphatase
UniProt Gene Name:	PPP2R2A
UniProt Entry Name:	2ABA_HUMAN

	Western blot analysis of extracts of various cell lines, using PPP2R2A antibody (CAB2185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat kidney using PPP2R2A antibody (CAB2185) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using PPP2R2A antibody (CAB2185) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using PPP2R2A antibody (CAB2185) at dilution of 1:100 (40x lens).