Signal Transduction Antibodies 1

Anti-PPME1 Antibody (CAB13094)

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SKU:
CAB13094
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-PPME1 Antibody
Antibody SKU:CAB13094
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PPME1 (NP_057231.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, Mouse testis, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human PPME1 (NP_057231.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSAL EKSM HLGR LPSR PPLP GSGG SQSG AKMR MGPG RKRD FSPV PWSQ YFES MEDV EVEN ETGK DTFR VYKS GSEG PVLL LLHG GGHS ALSW AVFT AAII SRVQ CRIV ALDL RSHG ETKV KNPE DLSA ETMA KDVG NVVE AMYG DLPP PIML IGHS MGGA
Gene ID:51400
Uniprot:Q9Y570
Cellular Location:
Calculated MW:18kDa/22kDa/42kDa/43kDa
Observed MW:42kDa
Synonyms:PPME1, PME-1
Background:This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PPME1: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme. Belongs to the AB hydrolase superfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Hydrolase; Motility/polarity/chemotaxis; EC 3.1.1.89; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q13.4

Molecular Function:protein C-terminal methylesterase activity; protein phosphatase 2A binding; protein phosphatase binding; protein phosphatase inhibitor activity; protein phosphatase type 2A regulator activity

Biological Process: protein amino acid demethylation

NCBI Summary:This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
UniProt Code:Q9Y570
NCBI GenInfo Identifier:47606055
NCBI Gene ID:51400
NCBI Accession:Q9Y570.3
UniProt Secondary Accession:Q9Y570,Q8WYG8, Q9NVT5, Q9UI18, B3KMU6, B5MEE7, J3QT22
UniProt Related Accession:Q9Y570
Molecular Weight:43,870 Da
NCBI Full Name:Protein phosphatase methylesterase 1
NCBI Synonym Full Names:protein phosphatase methylesterase 1
NCBI Official Symbol:PPME1  
NCBI Official Synonym Symbols:PME-1  
NCBI Protein Information:protein phosphatase methylesterase 1
UniProt Protein Name:Protein phosphatase methylesterase 1
Protein Family:Pectinesterase
UniProt Gene Name:PPME1  
UniProt Entry Name:PPME1_HUMAN
Anti-PPME1 Antibody (CAB13094)Western blot analysis of extracts of various cell lines, using PPME1 antibody (CAB13094) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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