Antibody Name:	Anti-PPM1A Antibody
Antibody SKU:	CAB6699
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PPM1A (NP_066283.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, HeLa, HepG2, SH-SY5Y, Mouse liver, Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PPM1A (NP_066283.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGAF LDKP KMEK HNAQ GQGN GLRY GLSS MQGW RVEM EDAH TAVI GLPS GLES WSFF AVYD GHAG SQVA KYCC EHLL DHIT NNQD FKGS AGAP SVEN VKNG IRTG FLEI DEHM RVMS EKKH GADR SGST AVGV LISP QHTY FINC GDSR GLLC RNRK VHFF TQDH KPSN PLEK ERIQ NAGG SVMI QRVN GSLA VSRA LGDF DYKC VHGK GPTE QLVS PEPE VHDI ERSE EDDQ FIIL ACDG IWDV MGNE ELCD FVRS RLEV TDDL EKVC NEVV DTCL YKGS RDNM SVIL ICFP NAPK VSPE AVKK EAEL DKYL ECRV EEII KKQG EGVP DLVH VMRT LASE NIPS LPPG GELA SKRN VIEA VYNR LNPY KNDD TDST STDD MW
Gene ID:	5494
Uniprot:	P35813
Cellular Location:	Cytoplasm, Lipid-anchor, Membrane, Nucleus, cytosol
Calculated MW:	35kDa/42kDa/51kDa
Observed MW:	42kDa

Synonyms:

PPM1A, PP2C-ALPHA, PP2CA, PP2Calpha

Background:

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
NCBI Summary:	The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P35813
NCBI GenInfo Identifier:	548442
NCBI Gene ID:	5494
NCBI Accession:	P35813.1
UniProt Secondary Accession:	P35813,O75551, B5BU11, J3KNM0,
UniProt Related Accession:	P35813
Molecular Weight:	51,366 Da
NCBI Full Name:	Protein phosphatase 1A
NCBI Synonym Full Names:	protein phosphatase, Mg2+/Mn2+ dependent 1A
NCBI Official Symbol:	PPM1A
NCBI Official Synonym Symbols:	PP2CA; PP2Calpha; PP2C-ALPHA
NCBI Protein Information:	protein phosphatase 1A
UniProt Protein Name:	Protein phosphatase 1A
UniProt Synonym Protein Names:	Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA
Protein Family:	Protein phosphatase
UniProt Gene Name:	PPM1A
UniProt Entry Name:	PPM1A_HUMAN

	Western blot analysis of extracts of various cell lines, using PPM1A antibody (CAB6699) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using PPM1A Rabbit pAb (CAB6699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using PPM1A Rabbit pAb (CAB6699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PPM1A Rabbit pAb (CAB6699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.