Name: Anti-PPIH Antibody (CAB12274)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB12274
Availability: OutOfStock

Antibody Name:	Anti-PPIH Antibody
Antibody SKU:	CAB12274
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human PPIH (NP_006338.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	HepG2, Rat testis, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human PPIH (NP_006338.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVA NSSP VNPV VFFD VSIG GQEV GRMK IELF ADVV PKTA ENFR QFCT GEFR KDGV PIGY KGST FHRV IKDF MIQG GDFV NGDG TGVA SIYR GPFA DENF KLRH SAPG LLSM ANSG PSTN GCQF FITC SKCD WLDG KHVV FGKI IDGL LVMR KIEN VPTG PNNK PKLP VVIS QCGE M
Gene ID:	10465
Uniprot:	O43447
Cellular Location:	Cytoplasm, Nucleus speckle
Calculated MW:	14kDa/19kDa
Observed MW:	19kDa

Synonyms:

PPIH, CYP-20, CYPH, SnuCyp-20, USA-CYP

Background:

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome.

UniProt Protein Function:	PPIH: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone. Belongs to the cyclophilin-type PPIase family. PPIase H subfamily.
UniProt Protein Details:	Protein type:Cyclophilin; EC 5.2.1.8; Isomerase; RNA processing; Spliceosome Chromosomal Location of Human Ortholog: 1p34.2 Cellular Component: cytoplasm; nuclear speck; nucleoplasm; spliceosome; U4/U6 x U5 tri-snRNP complex Molecular Function:cyclosporin A binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; ribonucleoprotein binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of viral genome replication; protein complex assembly
NCBI Summary:	The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]
UniProt Code:	O43447
NCBI GenInfo Identifier:	37082225
NCBI Gene ID:	10465
NCBI Accession:	O43447.1
UniProt Secondary Accession:	O43447,A6NNE7,
UniProt Related Accession:	O43447
Molecular Weight:	19kDa
NCBI Full Name:	Peptidyl-prolyl cis-trans isomerase H
NCBI Synonym Full Names:	peptidylprolyl isomerase H
NCBI Official Symbol:	PPIH
NCBI Official Synonym Symbols:	CYPH; CYP-20; USA-CYP; SnuCyp-20
NCBI Protein Information:	peptidyl-prolyl cis-trans isomerase H
UniProt Protein Name:	Peptidyl-prolyl cis-trans isomerase H
UniProt Synonym Protein Names:	Rotamase H; Small nuclear ribonucleoprotein particle-specific cyclophilin H; CypH; U-snRNP-associated cyclophilin SnuCyp-20; USA-CYP
UniProt Gene Name:	PPIH

Western blot analysis of extracts of various cell lines, using PPIH antibody (CAB12274) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

Anti-PPIH Antibody (CAB12172)

PPIH Antibody (PACO11400)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)