Epigenetics & Nuclear Signaling Antibodies 2

Anti-PPARD Antibody (CAB5656)

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SKU:
CAB5656

Description

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Antibody Name:Anti-PPARD Antibody
Antibody SKU:CAB5656
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 102-361 of human PPARD (NP_803184.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 102-361 of human PPARD (NP_803184.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IRMK LEYE KCER SCKI QKKN RNKC QYCR FQKC LALG MSHN AIRF GRMP EAEK RKLV AGLT ANEG SQYN PQVA DLKA FSKH IYNA YLKN FNMT KKKA RSIL TGKA SHTA PFVI HDIE TLWQ AEKG LVWK QLVN GLPP YKEI SVHV FYRC QCTT VETV RELT EFAK SIPS FSSL FLND QVTL LKYG VHEA IFAM LASI VNKD GLLV ANGS GFVT REFL RSLR KPFS DIIE PKFE FAVK FNAL ELDD SDLA LFIA AIIL CGGE
Gene ID:5467
Uniprot:Q03181
Cellular Location:Nucleus
Calculated MW:38kDa/40kDa/45kDa/49kDa
Observed MW:56kDa
Synonyms:PPARD, FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB
Background:This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
NCBI Summary:This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
UniProt Code:Q03181
NCBI GenInfo Identifier:417522
NCBI Gene ID:5467
NCBI Accession:Q03181.1
UniProt Secondary Accession:Q03181,Q5D1P0, Q7Z5K0, Q9BUD4, A8K6J6, B4E3V3, B6ZGS1 B7Z3W1, E9PE18,
UniProt Related Accession:Q03181
Molecular Weight:38,855 Da
NCBI Full Name:Peroxisome proliferator-activated receptor delta
NCBI Synonym Full Names:peroxisome proliferator activated receptor delta
NCBI Official Symbol:PPARD  
NCBI Official Synonym Symbols:FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB  
NCBI Protein Information:peroxisome proliferator-activated receptor delta
UniProt Protein Name:Peroxisome proliferator-activated receptor delta
UniProt Synonym Protein Names:NUCI; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; PPAR-beta
Protein Family:Peroxisome proliferator-activated receptor
UniProt Gene Name:PPARD  
UniProt Entry Name:PPARD_HUMAN
Anti-PPARD Antibody (CAB5656)Western blot analysis of extracts of various cell lines, using PPARD antibody (CAB5656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PPARD Antibody (CAB5656)Immunofluorescence analysis of U2OS cells using PPARD antibody (CAB5656). Blue: DAPI for nuclear staining.
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Additional Information

Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
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