Cell Biology Antibodies 8
Anti-POMC Antibody (CAB3038)
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- SKU:
- CAB3038
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-POMC Antibody |
| Antibody SKU: | CAB3038 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-267 of human POMC (NP_000930.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Raji, Mouse testis, Mouse skeletal muscle, Mouse intestine, Rat testis, Rat skeletal muscle, Rat intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-267 of human POMC (NP_000930.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPRS CCSR SGAL LLAL LLQA SMEV RGWC LESS QCQD LTTE SNLL ECIR ACKP DLSA ETPM FPGN GDEQ PLTE NPRK YVMG HFRW DRFG RRNS SSSG SSGA GQKR EDVS AGED CGPL PEGG PEPR SDGA KPGP REGK RSYS MEHF RWGK PVGK KRRP VKVY PNGA EDES AEAF PLEF KREL TGQR LREG DGPD GPAD DGAG AQAD LEHS LLVA AEKK DEGP YRME HFRW GSPP KDKR YGGF MTSE KSQT PLVT LFKN AIIK NAYK KGE |
| Gene ID: | 5443 |
| Uniprot: | P01189 |
| Cellular Location: | Secreted |
| Calculated MW: | 29kDa |
| Observed MW: | 29kDa |
| Synonyms: | POMC, ACTH, CLIP, LPH, MSH, NPP, POC |
| Background: | This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. |
| UniProt Protein Function: | POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: peroxisomal matrix; extracellular space; cytoplasm; extracellular region; peroxisome; secretory granule Molecular Function:type 3 melanocortin receptor binding; G-protein-coupled receptor binding; type 4 melanocortin receptor binding; hormone activity; receptor binding Biological Process: generation of precursor metabolites and energy; cellular protein metabolic process; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; peptide hormone processing; regulation of appetite; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; signal transduction; glucose homeostasis; cellular pigmentation Disease: Obesity; Proopiomelanocortin Deficiency |
| NCBI Summary: | This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Nov 2014] |
| UniProt Code: | P01189 |
| NCBI GenInfo Identifier: | 116880 |
| NCBI Gene ID: | 5443 |
| NCBI Accession: | P01189.2 |
| UniProt Secondary Accession: | P01189,P78442, Q53T23, Q9UD39, Q9UD40, |
| UniProt Related Accession: | P01189 |
| Molecular Weight: | 267 |
| NCBI Full Name: | Pro-opiomelanocortin |
| NCBI Synonym Full Names: | proopiomelanocortin |
| NCBI Official Symbol: | POMC |
| NCBI Official Synonym Symbols: | LPH; MSH; NPP; POC; ACTH; CLIP |
| NCBI Protein Information: | pro-opiomelanocortin; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; adrenocorticotropin; corticotropin-lipotropin; adrenocorticotropic hormone; opiomelanocortin prepropeptide; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide |
| UniProt Protein Name: | Pro-opiomelanocortin |
| UniProt Synonym Protein Names: | Corticotropin-lipotropinCleaved into the following 11 chains:NPP; Melanotropin gammaAlternative name(s):Gamma-MSH |
| Protein Family: | Pro-opiomelanocortin |
| UniProt Gene Name: | POMC |
| UniProt Entry Name: | COLI_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POMC antibody (CAB3038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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