Epigenetics & Nuclear Signaling Antibodies 3
Anti-POLR2J Antibody (CAB1843)
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- SKU:
- CAB1843
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLR2J Antibody |
| Antibody SKU: | CAB1843 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | K-562, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNAP PAFE SFLL FEGE KKIT INKD TKVP NACL FTIN KEDH TLGN IIKS QLLK DPQV LFAG YKVP HPLE HKII IRVQ TTPD YSPQ EAFT NAIT DLIS ELSL LEER FRVA IKDK QEGI E |
| Gene ID: | 5439 |
| Uniprot: | P52435 |
| Cellular Location: | Nucleus |
| Calculated MW: | 13kDa |
| Observed MW: | 16kDa |
| Synonyms: | POLR2J, POLR2J1, RPB11, RPB11A, RPB11m, hRPB14 |
| Background: | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. |
| UniProt Protein Function: | POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:Transferase; DNA-binding; RNA splicing; RNA processing Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus Molecular Function:DNA-directed RNA polymerase activity; LRR domain binding; protein binding Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair |
| NCBI Summary: | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52435 |
| NCBI GenInfo Identifier: | 1710661 |
| NCBI Gene ID: | 5439 |
| NCBI Accession: | P52435.1 |
| UniProt Secondary Accession: | P52435,O43375, A5D6V8, |
| UniProt Related Accession: | P52435 |
| Molecular Weight: | 13,293 Da |
| NCBI Full Name: | DNA-directed RNA polymerase II subunit RPB11-a |
| NCBI Synonym Full Names: | RNA polymerase II subunit J |
| NCBI Official Symbol: | POLR2J |
| NCBI Official Synonym Symbols: | RPB11; RPB11A; RPB11m; hRPB14; POLR2J1 |
| NCBI Protein Information: | DNA-directed RNA polymerase II subunit RPB11-a |
| UniProt Protein Name: | DNA-directed RNA polymerase II subunit RPB11-a |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR2J |
| UniProt Entry Name: | RPB11_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLR2J antibody (CAB1843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using POLR2J antibody (CAB1843). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of A549 cells using POLR2J antibody (CAB1843). |
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