Name: Anti-POLR2J Antibody (CAB13627)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB13627
Availability: OutOfStock

Antibody Name:	Anti-POLR2J Antibody
Antibody SKU:	CAB13627
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNAP PAFE SFLL FEGE KKIT INKD TKVP NACL FTIN KEDH TLGN IIKS QLLK DPQV LFAG YKVP HPLE HKII IRVQ TTPD YSPQ EAFT NAIT DLIS ELSL LEER FRVA IKDK QEGI E
Gene ID:	5439
Uniprot:	P52435
Cellular Location:	Nucleus
Calculated MW:	13kDa
Observed MW:	13kDa

Synonyms:	POLR2J, POLR2J1, RPB11, RPB11A, RPB11m, hRPB14
Background:	This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.

UniProt Protein Function:	POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family.
UniProt Protein Details:	Protein type:Transferase; DNA-binding; RNA splicing; RNA processing Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus Molecular Function:DNA-directed RNA polymerase activity; LRR domain binding; protein binding Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair
NCBI Summary:	This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]
UniProt Code:	P52435
NCBI GenInfo Identifier:	1710661
NCBI Gene ID:	5439
NCBI Accession:	P52435.1
UniProt Secondary Accession:	P52435,O43375, A5D6V8,
UniProt Related Accession:	P52435
Molecular Weight:	13,293 Da
NCBI Full Name:	DNA-directed RNA polymerase II subunit RPB11-a
NCBI Synonym Full Names:	RNA polymerase II subunit J
NCBI Official Symbol:	POLR2J
NCBI Official Synonym Symbols:	RPB11; RPB11A; RPB11m; hRPB14; POLR2J1
NCBI Protein Information:	DNA-directed RNA polymerase II subunit RPB11-a
UniProt Protein Name:	DNA-directed RNA polymerase II subunit RPB11-a
UniProt Synonym Protein Names:	DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit
Protein Family:	DNA-directed RNA polymerase
UniProt Gene Name:	POLR2J
UniProt Entry Name:	RPB11_HUMAN

	Western blot analysis of extracts of mouse skeletal muscle, using POLR2J antibody (CAB13627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using POLR2J antibody (CAB13627).