Epigenetics & Nuclear Signaling Antibodies 1
Anti-POLR1E Antibody (CAB12700)
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- SKU:
- CAB12700
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLR1E Antibody |
| Antibody SKU: | CAB12700 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1). |
| Application: | WB IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, 293T, Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human POLR1E (NP_071935.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAE VLPS ARWQ YCGA PDGS QRAV LVQF SNGK LQSP GNMR FTLY ENKD STNP RKRN QRIL AAET DRLS YVGN NFGT GALK CNTL CRHF VGIL NKTS GQME VYDA ELFN MQPL FSDV SVES ELAL ESQT KTYR EKMD SCIE AFGT TKQK RALN TRRM NRVG NESL NRAV AKAA ETII DTKG VTAL VSDA IHND LQDD SLYL PPCY DDAA KPED VYKF EDLL SPAE YEAL QSPS EAFR NVTS EEIL KMIE ENSH CTFV IEAL KSLP SDVE SRDR QARC IWFL DTLI KFRA HRVV KRKS ALGP GVPH IINT KLLK HFTC LTYN NGRL RNLI SDSM KAKI TAYV IILA LHIH DFQI DLTV LQRD LKLS EKRM MEIA KAMR LKIS KRRV SVAA GSEE DHKL GTLS LPLP PAQT SDRL AKRR KIT |
| Gene ID: | 64425 |
| Uniprot: | Q9GZS1 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 47kDa/53kDa |
| Observed MW: | 53kDa |
| Synonyms: | POLR1E, PAF53, PRAF1 |
| Background: |
| UniProt Protein Function: | POLR1E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF. Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleolus; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 9p13.2 Cellular Component: nucleoplasm; nucleolus; nucleus; DNA-directed RNA polymerase I complex Molecular Function:DNA binding Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic; rRNA transcription |
| UniProt Code: | Q9GZS1 |
| NCBI GenInfo Identifier: | 62901107 |
| NCBI Gene ID: | 64425 |
| NCBI Accession: | Q9GZS1.2 |
| UniProt Secondary Accession: | Q9GZS1,Q5VZT3, Q8NBA9, Q96L20, |
| UniProt Related Accession: | Q9GZS1 |
| Molecular Weight: | 481 |
| NCBI Full Name: | DNA-directed RNA polymerase I subunit RPA49 |
| NCBI Synonym Full Names: | polymerase (RNA) I polypeptide E, 53kDa |
| NCBI Official Symbol: | POLR1E |
| NCBI Official Synonym Symbols: | PAF53; PRAF1 |
| NCBI Protein Information: | DNA-directed RNA polymerase I subunit RPA49; RNA polymerase I subunit A49; RNA polymerase I-associated factor 53; polymerase (RNA) I associated factor 1; DNA-directed RNA polymerase I subunit E |
| UniProt Protein Name: | DNA-directed RNA polymerase I subunit RPA49 |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase I subunit E; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53 |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR1E |
| UniProt Entry Name: | RPA49_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLR1E antibody (CAB12700) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug POLR1E antibody (CAB12700). Western blot was performed from the immunoprecipitate using POLR1E antibody (CAB12700) at a dilition of 1:1000. |
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