Epigenetics & Nuclear Signaling Antibodies 1
Anti-POLR1C Antibody (CAB0269)
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- SKU:
- CAB0269
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLR1C Antibody |
| Antibody SKU: | CAB0269 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR1C (NP_976035.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | K-562, 293T, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR1C (NP_976035.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS QAVE EMRS RVVL GEFG VRNV HTTD FPGN YSGY DDAW DQDR FEKN FRVD VVHM DENS LEFD MVGI DAAI ANAF RRIL LAEV PTMA VEKV LVYN NTSI VQDE ILAH RLGL IPIH ADPR LFEY RNQG DEEG TEID TLQF RLQV RCTR NPHA AKDS SDPN ELYV NHKV YTRH MTWI PLGN QADL FPEG TIRP VHDD ILIA |
| Gene ID: | 9533 |
| Uniprot: | O15160 |
| Cellular Location: | Nucleus |
| Calculated MW: | 38kDa/39kDa |
| Observed MW: | 39kDa |
| Synonyms: | POLR1C, AC40, HLD11, RPA39, RPA40, RPA5, RPAC1, RPC40, TCS3 |
| Background: | The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Mutations in this gene have been associated with Treacher Collins syndrome (TCS) and hypomyelinating leukodystrophy 11. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | RPA40: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Defects in POLR1C are the cause of Treacher Collins syndrome type 3 (TCS3). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transcription initiation complex Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytosol; DNA-directed RNA polymerase I complex Molecular Function:protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; negative regulation of gene expression, epigenetic; positive regulation of interferon type I production; transcription from RNA polymerase I promoter; innate immune response; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; RNA elongation from RNA polymerase III promoter Disease: Leukodystrophy, Hypomyelinating, 11; Treacher Collins Syndrome 3 |
| NCBI Summary: | The protein encoded by this gene is a subunit of both RNA polymerase I and RNA polymerase III complexes. The encoded protein is part of the Pol core element. Defects in this gene have been associated with Treacher Collins syndrome (TCS). [provided by RefSeq, Mar 2011] |
| UniProt Code: | O15160 |
| NCBI GenInfo Identifier: | 3914807 |
| NCBI Gene ID: | 9533 |
| NCBI Accession: | O15160.1 |
| UniProt Secondary Accession: | O15160,O75395, Q5JTE3, |
| UniProt Related Accession: | O15160 |
| Molecular Weight: | 346 |
| NCBI Full Name: | DNA-directed RNA polymerases I and III subunit RPAC1 |
| NCBI Synonym Full Names: | polymerase (RNA) I polypeptide C, 30kDa |
| NCBI Official Symbol: | POLR1C |
| NCBI Official Synonym Symbols: | RPA5; TCS3; RPA39; RPA40; RPAC1 |
| NCBI Protein Information: | DNA-directed RNA polymerases I and III subunit RPAC1; AC40; RPC40; RNA polymerases I and III subunit AC1; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide |
| UniProt Protein Name: | DNA-directed RNA polymerases I and III subunit RPAC1 |
| UniProt Synonym Protein Names: | AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40 |
| Protein Family: | DNA-directed RNA polymerases |
| UniProt Gene Name: | POLR1C |
| UniProt Entry Name: | RPAC1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLR1C antibody (CAB0269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using POLR1C antibody (CAB0269). Blue: DAPI for nuclear staining. |
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