Epigenetics & Nuclear Signaling Antibodies 2
Anti-POLK Antibody (CAB6122)
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- SKU:
- CAB6122
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLK Antibody |
| Antibody SKU: | CAB6122 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLK (NP_057302.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, Mouse kidney, Mouse lung, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLK (NP_057302.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDST KEKC DSYK DDLL LRMG LNDN KAGM EGLD KEKI NKII MEAT KGSR FYGN ELKK EKQV NQRI ENMM QQKA QITS QQLR KAQL QVDR FAME LEQS RNLS NTIV HIDM DAFY AAVE MRDN PELK DKPI AVGS MSML STSN YHAR RFGV RAAM PGFI AKRL CPQL IIVP PNFD KYRA VSKE VKEI LADY DPNF MAMS LDEA YLNI TKHL EERQ NWPE DKRR YFIK MGSS VEND NPGK EVNK LSEH ERSI SPLL FEES PSDV |
| Gene ID: | 51426 |
| Uniprot: | Q9UBT6 |
| Cellular Location: | Nucleus |
| Calculated MW: | 42kDa/52kDa/54kDa/55kDa/76kDa/88kDa/98kDa |
| Observed MW: | 80kDa/110kDa |
| Synonyms: | POLK, DINB1, DINP, POLQ |
| Background: |
| UniProt Protein Function: | POLK: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Belongs to the DNA polymerase type-Y family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage; Transferase; EC 2.7.7.7; DNA replication Chromosomal Location of Human Ortholog: 5q13 Cellular Component: nucleoplasm Molecular Function:metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding Biological Process: nucleotide-excision repair, DNA gap filling; DNA repair; DNA replication |
| NCBI Summary: | External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010] |
| UniProt Code: | Q9UBT6 |
| NCBI GenInfo Identifier: | 59798438 |
| NCBI Gene ID: | 51426 |
| NCBI Accession: | Q9UBT6.1 |
| UniProt Secondary Accession: | Q9UBT6,Q5Q9G5, Q5Q9G6, Q5Q9G7, Q5Q9G8, Q86VJ8, Q8IZY0 Q8IZY1, Q8NB30, Q96L01, Q96Q86, B2RBD2, |
| UniProt Related Accession: | Q9UBT6 |
| Molecular Weight: | 870 |
| NCBI Full Name: | DNA polymerase kappa |
| NCBI Synonym Full Names: | polymerase (DNA directed) kappa |
| NCBI Official Symbol: | POLK |
| NCBI Official Synonym Symbols: | DINP; POLQ; DINB1 |
| NCBI Protein Information: | DNA polymerase kappa |
| UniProt Protein Name: | DNA polymerase kappa |
| UniProt Synonym Protein Names: | DINB protein; DINP |
| UniProt Gene Name: | POLK |
| UniProt Entry Name: | POLK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLK antibody (CAB6122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLK antibody (CAB6122). (Green)GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region. |
 | Immunofluorescence analysis of U2OS cells using POLK antibody (CAB6122). Blue: DAPI for nuclear staining. |
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