Epigenetics & Nuclear Signaling Antibodies 1

Anti-POLK Antibody (CAB12333)

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SKU:
CAB12333
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-POLK Antibody
Antibody SKU:CAB12333
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POLK (NP_057302.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse heart, Mouse brain, Mouse kidney, Rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POLK (NP_057302.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDST KEKC DSYK DDLL LRMG LNDN KAGM EGLD KEKI NKII MEAT KGSR FYGN ELKK EKQV NQRI ENMM QQKA QITS QQLR KAQL QVDR FAME LEQS RNLS
Gene ID:51426
Uniprot:Q9UBT6
Cellular Location:Nucleus
Calculated MW:42kDa/52kDa/54kDa/55kDa/76kDa/88kDa/98kDa
Observed MW:99kDa
Synonyms:POLK, DINB1, DINP, POLQ
Background:
UniProt Protein Function:POLK: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Belongs to the DNA polymerase type-Y family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA repair, damage; Transferase; EC 2.7.7.7; DNA replication

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: nucleoplasm

Molecular Function:metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding

Biological Process: nucleotide-excision repair, DNA gap filling; DNA repair; DNA replication

NCBI Summary:External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010]
UniProt Code:Q9UBT6
NCBI GenInfo Identifier:59798438
NCBI Gene ID:51426
NCBI Accession:Q9UBT6.1
UniProt Secondary Accession:Q9UBT6,Q5Q9G5, Q5Q9G6, Q5Q9G7, Q5Q9G8, Q86VJ8, Q8IZY0 Q8IZY1, Q8NB30, Q96L01, Q96Q86, B2RBD2,
UniProt Related Accession:Q9UBT6
Molecular Weight:870
NCBI Full Name:DNA polymerase kappa
NCBI Synonym Full Names:polymerase (DNA directed) kappa
NCBI Official Symbol:POLK  
NCBI Official Synonym Symbols:DINP; POLQ; DINB1  
NCBI Protein Information:DNA polymerase kappa
UniProt Protein Name:DNA polymerase kappa
UniProt Synonym Protein Names:DINB protein; DINP
UniProt Gene Name:POLK  
UniProt Entry Name:POLK_HUMAN
Anti-POLK Antibody (CAB12333)Western blot analysis of extracts of various cell lines, using POLK antibody (CAB12333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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