Epigenetics & Nuclear Signaling Antibodies 1
Anti-POLK Antibody (CAB12052)
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- SKU:
- CAB12052
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-POLK Antibody |
| Antibody SKU: | CAB12052 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POLK (NP_057302.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 22RV1, SKOV3, U-87MG, Mouse liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POLK (NP_057302.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDST KEKC DSYK DDLL LRMG LNDN KAGM EGLD KEKI NKII MEAT KGSR FYGN ELKK EKQV NQRI ENMM QQKA QITS QQLR KAQL QVDR FAME LEQS RNLS |
| Gene ID: | 51426 |
| Uniprot: | Q9UBT6 |
| Cellular Location: | Nucleus |
| Calculated MW: | 42kDa/52-55kDa/76kDa/88kDa/98kDa |
| Observed MW: | 71kDa |
| Synonyms: | POLK, DINB1, DINP, POLQ |
| Background: |
| UniProt Protein Function: | POLK: DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Belongs to the DNA polymerase type-Y family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA repair, damage; Transferase; EC 2.7.7.7; DNA replication Chromosomal Location of Human Ortholog: 5q13 Cellular Component: nucleoplasm Molecular Function:metal ion binding; DNA-directed DNA polymerase activity; damaged DNA binding Biological Process: nucleotide-excision repair, DNA gap filling; DNA repair; DNA replication |
| NCBI Summary: | External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass (summary by Lone et al., 2007 [PubMed 17317631]).[supplied by OMIM, Jan 2010] |
| UniProt Code: | Q9UBT6 |
| NCBI GenInfo Identifier: | 59798438 |
| NCBI Gene ID: | 51426 |
| NCBI Accession: | Q9UBT6.1 |
| UniProt Secondary Accession: | Q9UBT6,Q5Q9G5, Q5Q9G6, Q5Q9G7, Q5Q9G8, Q86VJ8, Q8IZY0 Q8IZY1, Q8NB30, Q96L01, Q96Q86, B2RBD2, |
| UniProt Related Accession: | Q9UBT6 |
| Molecular Weight: | 870 |
| NCBI Full Name: | DNA polymerase kappa |
| NCBI Synonym Full Names: | polymerase (DNA directed) kappa |
| NCBI Official Symbol: | POLK |
| NCBI Official Synonym Symbols: | DINP; POLQ; DINB1 |
| NCBI Protein Information: | DNA polymerase kappa |
| UniProt Protein Name: | DNA polymerase kappa |
| UniProt Synonym Protein Names: | DINB protein; DINP |
| UniProt Gene Name: | POLK |
| UniProt Entry Name: | POLK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using POLK antibody (CAB12052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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