Name: Anti-POLG2 Antibody (CAB6695)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB6695
Availability: OutOfStock

Antibody Name:	Anti-POLG2 Antibody
Antibody SKU:	CAB6695
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HPVF DTKQ IRNG VKSI GEKT EASL VWFT PPRT SNQW LDFW LRHR LQWW RKFA MSPS NFSS SDCQ DEEG RKGN KLYY NFPW GKEL IETL WNLG DHEL LHMY PGNV SKLH GRDG RKNV VPCV LSVN GDLD RGML AYLY DSFQ LTEN SFTR KKNL HRKV LKLH PCLA PIKV ALDV GRGP TLEL RQVC QGLF NELL ENGI SVWP GYLE TMQS SLEQ LYSK YDEM SILF TVLV TETT LENG LIHL RSRD TTMK EMMH ISKL KDFL IKYI SSAK NV
Gene ID:	11232
Uniprot:	Q9UHN1
Cellular Location:	Mitochondrion
Calculated MW:	54kDa
Observed MW:	55kDa

Synonyms:	POLG2, HP55, MTPOLB, PEOA4, POLB, POLG-BETA, POLGB
Background:	This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.

UniProt Protein Function:	POLG2: Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA. Defects in POLG2 are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 4 (PEOA4). Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged- red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
UniProt Protein Details:	Protein type:DNA replication; EC 2.7.7.7; Transferase; Mitochondrial; DNA repair, damage Chromosomal Location of Human Ortholog: 17q Cellular Component: mitochondrial matrix Molecular Function:glycine-tRNA ligase activity; protein binding Biological Process: DNA-dependent DNA replication; glycyl-tRNA aminoacylation; mitochondrion organization and biogenesis Disease: Progressive External Ophthalmoplegia With Mitochondrial Dna Deletions, Autosomal Dominant, 4
NCBI Summary:	This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.[provided by RefSeq, Sep 2009]
UniProt Code:	Q9UHN1
NCBI GenInfo Identifier:	17367139
NCBI Gene ID:	11232
NCBI Accession:	Q9UHN1.1
UniProt Secondary Accession:	Q9UHN1,O00419, Q0IJ81, Q96GW2, Q9UK35, Q9UK94,
UniProt Related Accession:	Q9UHN1
Molecular Weight:	54,911 Da
NCBI Full Name:	DNA polymerase subunit gamma-2, mitochondrial
NCBI Synonym Full Names:	DNA polymerase gamma 2, accessory subunit
NCBI Official Symbol:	POLG2
NCBI Official Synonym Symbols:	HP55; POLB; PEOA4; POLGB; MTPOLB; POLG-BETA
NCBI Protein Information:	DNA polymerase subunit gamma-2, mitochondrial
UniProt Protein Name:	DNA polymerase subunit gamma-2, mitochondrial
UniProt Synonym Protein Names:	DNA polymerase gamma accessory 55 kDa subunit; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta
Protein Family:	DNA polymerase
UniProt Gene Name:	POLG2
UniProt Entry Name:	DPOG2_HUMAN

	Western blot analysis of extracts of various cell lines, using POLG2 antibody (CAB6695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using POLG2 antibody (CAB6695) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using POLG2 antibody (CAB6695) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using POLG2 antibody (CAB6695) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using POLG2 antibody (CAB6695) at dilution of 1:100 (40x lens).