Metabolism Antibodies 1

Anti-PMVK Antibody (CAB13865)

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SKU:
CAB13865
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PMVK Antibody
Antibody SKU:CAB13865
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human PMVK (NP_006547.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, LO2, A-431, HeLa, Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human PMVK (NP_006547.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAPL GGAP RLVL LFSG KRKS GKDF VTEA LQSR LGAD VCAV LRLS GPLK EQYA QEHG LNFQ RLLD TSTY KEAF RKDM IRWG EEKR QADP GFFC RKIV EGIS QPIW LVSD TRRV SDIQ WFRE AYGA VTQT VRVV ALEQ SRQQ RGWV FTPG VDDA ESEC GLDN FGDF DWVI ENHG VEQR LEEQ LENL IEFI RSRL
Gene ID:10654
Uniprot:Q15126
Cellular Location:Peroxisome
Calculated MW:21kDa
Observed MW:22kDa
Synonyms:PMVK, HUMPMKI, PMK, PMKA, PMKASE, POROK1
Background:This gene encodes a peroxisomal enzyme that is a member of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family of ATP-dependent enzymes. The encoded protein catalyzes the conversion of mevalonate 5-phosphate to mevalonate 5-diphosphate, which is the fifth step in the mevalonate pathway of isoprenoid biosynthesis. Mutations in this gene are linked to certain types of porokeratosis including disseminated superficial porokeratosis. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:PMVK: Induced by sterol. Monomer.
UniProt Protein Details:

Protein type:Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.7.4.2; Kinase, other

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: membrane; peroxisome; cytosol

Molecular Function:phosphomevalonate kinase activity; ATP binding

Biological Process: isopentenyl diphosphate biosynthetic process, mevalonate pathway; sterol biosynthetic process; phosphorylation; cholesterol biosynthetic process

NCBI Summary:This gene encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. [provided by RefSeq, Sep 2011]
UniProt Code:Q15126
NCBI GenInfo Identifier:3024422
NCBI Gene ID:10654
NCBI Accession:Q15126.3
UniProt Secondary Accession:Q15126,Q5TZW9,
UniProt Related Accession:Q15126
Molecular Weight:21,995 Da
NCBI Full Name:Phosphomevalonate kinase
NCBI Synonym Full Names:phosphomevalonate kinase
NCBI Official Symbol:PMVK  
NCBI Official Synonym Symbols:PMK; PMKA; PMKASE; HUMPMKI  
NCBI Protein Information:phosphomevalonate kinase; hPMK
UniProt Protein Name:Phosphomevalonate kinase
UniProt Gene Name:PMVK  
UniProt Entry Name:PMVK_HUMAN
Anti-PMVK Antibody (CAB13865)Western blot analysis of extracts of various cell lines, using PMVK antibody (CAB13865) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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