Cell Cycle Antibodies 1
Anti-PMF1 Antibody (CAB17095)
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- SKU:
- CAB17095
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-PMF1 Antibody |
| Antibody SKU: | CAB17095 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PMF1 (NP_009152.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human PMF1 (NP_009152.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEA SSAN LGSG CEEK RHEG SSSE SVPP GTTI SRVK LLDT MVDT FLQK LVAA GSYQ RFTD CYKC FYQL QPAM TQQI YDKF IAQL QTSI REEI SDIK EEGN LEAV LNAL DKIV EEGK VRKE PAWR PSGI PEKD LHSV MAPY FLQQ RDTL RRHV QKQE AENQ QLAD AVLA GRRQ VEEL QLQV QAQQ QAWQ ALHR EQRE LVAV LREP E |
| Gene ID: | 11243 |
| Uniprot: | Q6P1K2 |
| Cellular Location: | |
| Calculated MW: | |
| Observed MW: | Refer to figures |
| Synonyms: | PMF1 |
| Background: |
| UniProt Protein Function: | Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT. |
| NCBI Summary: | This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. [provided by RefSeq, Dec 2010] |
| UniProt Code: | Q6P1K2 |
| NCBI GenInfo Identifier: | 114152119 |
| NCBI Gene ID: | 100527963 |
| NCBI Accession: | Q6P1K2.2 |
| UniProt Secondary Accession: | Q6P1K2,Q5TCJ8, Q5TCJ9, Q5TCK0, Q5TCK1, Q5TCK3, Q69YZ9 Q6PHR4, Q6ZVE6, Q86VJ6, Q8N4T6, A8K0C5, |
| Molecular Weight: | 20,230 Da |
| NCBI Full Name: | Polyamine-modulated factor 1 |
| NCBI Synonym Full Names: | PMF1-BGLAP readthrough |
| NCBI Official Symbol: | PMF1-BGLAP |
| NCBI Official Synonym Symbols: | PMF1 |
| NCBI Protein Information: | PMF1-BGLAP protein |
| UniProt Protein Name: | Polyamine-modulated factor 1 |
| Protein Family: | Polyamine-modulated factor |
| UniProt Gene Name: | PMF1 |
| UniProt Entry Name: | PMF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PMF1 antibody (CAB17095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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