Developmental Biology
Anti-PMCH Antibody (CAB6692)
- SKU:
- CAB6692
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-PMCH Antibody |
| Antibody SKU: | CAB6692 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-165 of human PMCH (NP_002665.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HT-29, MCF7, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-165 of human PMCH (NP_002665.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ILLS ASKS IRNL DDDM VFNT FRLG KGFQ KEDT AEKS VIAP SLEQ YKND ESSF MNEE ENKV SKNT GSKH NFLN HGLP LNLA IKPY LALK GSVA FPAE NGVQ NTES TQEK REIG DEEN SAKF PIGR RDFD MLRC MLGR VYRP CWQV |
| Gene ID: | 5367 |
| Uniprot: | P20382 |
| Cellular Location: | Secreted |
| Calculated MW: | 18kDa |
| Observed MW: | 19kDa |
| Synonyms: | PMCH, MCH, ppMCH, pro-MCH |
| Background: | This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include melanin-concentrating hormone (MCH), neuropeptide-glutamic acid-isoleucine (NEI), and neuropeptide-glycine-glutamic acid (NGE). Melanin-concentrating hormone is a 19-amino acid neuropeptide that stimulates hunger and may additionally regulate energy homeostasis, reproductive function, and sleep. Pseudogenes of this gene have been identified on chromosome 5. |
| UniProt Protein Function: | PMCH: MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. May also have a role in spermatocyte differentiation. Belongs to the melanin-concentrating hormone family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 12q23-q24 |
| NCBI Summary: | The melanin-concentrating hormone (MCH) is a cyclic neuropeptide isolated initially from salmon pituitary gland and later from rat hypothalamus. In mammals, MCH perikarya are confined largely to the lateral hypothalamus and zona incerta area with extensive neuronal projections throughout the brain, including the neurohypophysis. The anatomic distribution suggests a neurotransmitter or neuromodulator role for MCH in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal. MCH and 2 other putative neuropeptides, NEI and NGE, are encoded by the same precursor and appear colocalized in nerve cells and in many instances within the projections. The precursor is designated pro-melanin-concentrating hormone (PMCH) (summary by Nahon et al., 1992 [PubMed 1572663]).[supplied by OMIM, Jan 2011] |
| UniProt Code: | P20382 |
| NCBI GenInfo Identifier: | 229462960 |
| NCBI Gene ID: | 5367 |
| NCBI Accession: | P20382.3 |
| UniProt Secondary Accession: | P20382,Q16044, Q8WVG0, |
| UniProt Related Accession: | P20382 |
| Molecular Weight: | 165 |
| NCBI Full Name: | Pro-MCH |
| NCBI Synonym Full Names: | pro-melanin-concentrating hormone |
| NCBI Official Symbol: | PMCH |
| NCBI Official Synonym Symbols: | MCH |
| NCBI Protein Information: | pro-MCH |
| UniProt Protein Name: | Pro-MCH |
| Protein Family: | Pro-MCH |
| UniProt Gene Name: | PMCH |
| UniProt Entry Name: | MCH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PMCH antibody (CAB6692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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