KO Validated Antibodies 2

Anti-PLS3 Antibody (CAB19956)[KO Validated]

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SKU:
CAB19956
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-PLS3 Antibody [KO Validated]
Antibody SKU:CAB19956
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human PLS3.
Application:WB IHC IP
Recommended Dilution:WB 1:200 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human PLS3.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5358
Uniprot:P13797
Cellular Location:Cytoplasm
Calculated MW:65kDa/69kDa/70kDa
Observed MW:68KDa
Anti-PLS3 Antibody [KO Validated] (CAB19956)Western blot analysis of extracts from normal (control) and PLS3 knockout (KO) 293T cells, using PLS3 antibody (CAB19956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:PLS3, BMND18, T-plastin, plastin-3
Background:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.
UniProt Protein Details:

Protein type:Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm

Molecular Function:calcium ion binding; actin binding

Disease: Bone Mineral Density Quantitative Trait Locus 18

UniProt Code:P13797
NCBI GenInfo Identifier:226694201
NCBI Gene ID:
NCBI Accession:P13797.4
Molecular Weight:~ 71kDa
NCBI Full Name:Plastin-3
UniProt Protein Name:Plastin-3
UniProt Synonym Protein Names:T-plastin
Protein Family:Plastin
UniProt Gene Name:PLS3  
UniProt Entry Name:PLST_HUMAN
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