Antibody Name:	Anti-PLIN3 Antibody
Antibody SKU:	CAB1050
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 135-434 of human PLIN3 (NP_005808.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HeLa, SW480, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 135-434 of human PLIN3 (NP_005808.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVSS AKDT VATQ LSEA VDAT RGAV QSGV DKTK SVVT GGVQ SVMG SRLG QMVL SGVD TVLG KSEE WADN HLPL TDAE LARI ATSL DGFD VASV QQQR QEQS YFVR LGSL SERL RQHA YEHS LGKL RATK QRAQ EALL QLSQ VLSL METV KQGV DQKL VEGQ EKLH QMWL SWNQ KQLQ GPEK EPPK PEQV ESRA LTMF RDIA QQLQ ATCT SLGS SIQG LPTN VKDQ VQQA RRQV EDLQ ATFS SIHS FQDL SSSI LAQS RERV ASAR EALD HMVE YVAQ NTPV TWLV GPFA PGIT EKAP EEKK
Gene ID:	10226
Uniprot:	O60664
Cellular Location:	Cytoplasm, Cytoplasmic side, Endosome membrane, Lipid droplet, Peripheral membrane protein
Calculated MW:	28kDa/45kDa/46kDa/47kDa
Observed MW:	47kDa

Synonyms:

PLIN3, M6PRBP1, PP17, TIP47

Background:

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	perilipin 3: a membrane-associated protein and a coat protein for lipid droplets (LDs). It has been described as a mannose-6-phosphate receptor (MPR) binding protein required for endosome-to-Golgi transport of MPRs, but this function has been disputed. Interacts with M6PR and IGF2R via their cytoplasmic domains. Affects hepatic lipid and glucose metabolism. Belongs to the perilipin family. 3 isoforms of the human protein are produced by alternative splicing. Isoform 2 may exist as a homodimer known as PP17C.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; cytoplasm; lipid particle; endosome membrane; endosome Molecular Function:protein binding Biological Process: vesicle-mediated transport
NCBI Summary:	Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]
UniProt Code:	O60664
NCBI GenInfo Identifier:	317373524
NCBI Gene ID:	10226
NCBI Accession:	O60664.3
UniProt Secondary Accession:	O60664,Q53G77, Q9BS03, Q9UBD7, Q9UP92, A8K4Y9, K7EQF4
UniProt Related Accession:	O60664
Molecular Weight:
NCBI Full Name:	Perilipin-3
NCBI Synonym Full Names:	perilipin 3
NCBI Official Symbol:	PLIN3
NCBI Official Synonym Symbols:	PP17; TIP47; M6PRBP1
NCBI Protein Information:	perilipin-3; placental protein 17; 47 kDa MPR-binding protein; cargo selection protein TIP47; tail-interacting protein, 47 kD; mannose-6-phosphate receptor-binding protein 1
UniProt Protein Name:	Perilipin-3
UniProt Synonym Protein Names:	47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17
Protein Family:	Perilipin
UniProt Gene Name:	PLIN3
UniProt Entry Name:	PLIN3_HUMAN

	Western blot analysis of extracts of various cell lines, using PLIN3 antibody (CAB1050) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human liver cancer using PLIN3 antibody (CAB1050) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using PLIN3 antibody (CAB1050) at dilution of 1:100 (40x lens).