Cell Biology Antibodies 10

Anti-PLIN2 Antibody (CAB6276)

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SKU:
CAB6276
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-PLIN2 Antibody
Antibody SKU:CAB6276
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human PLIN2 (NP_001113.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, NIH/3T3, Mouse brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 168-437 of human PLIN2 (NP_001113.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMQL VSSG VENA LTKS ELLV EQYL PLTE EELE KEAK KVEG FDLV QKPS YYVR LGSL STKL HSRA YQQA LSRV KEAK QKSQ QTIS QLHS TVHL IEFA RKNV YSAN QKIQ DAQD KLYL SWVE WKRS IGYD DTDE SHCA EHIE SRTL AIAR NLTQ QLQT TCHT LLSN IQGV PQNI QDQA KHMG VMAG DIYS VFRN AASF KEVS DSLL TSSK GQLQ KMKE SLDD VMDY LVNN TPLN WLVG PFYP QLTE SQNA QDQG AEMD KSSQ ETQR SEHK TH
Gene ID:123
Uniprot:Q99541
Cellular Location:Membrane, Peripheral membrane protein
Calculated MW:48kDa
Observed MW:48kDa
Synonyms:PLIN2, ADFP, ADRP
Background:The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.
UniProt Protein Details:

Protein type:Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; extracellular region; plasma membrane; lipid particle; nucleus; cytosol

Biological Process: long-chain fatty acid transport; response to drug; sequestering of lipid; cellular lipid metabolic process; response to organic cyclic substance

NCBI Summary:The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Code:Q99541
NCBI GenInfo Identifier:34577059
NCBI Gene ID:123
NCBI Accession:NP_001113.2
UniProt Secondary Accession:Q99541,Q9BSC3,
UniProt Related Accession:Q99541
Molecular Weight:48,075 Da
NCBI Full Name:perilipin-2
NCBI Synonym Full Names:perilipin 2
NCBI Official Symbol:PLIN2  
NCBI Official Synonym Symbols:ADFP; ADRP  
NCBI Protein Information:perilipin-2; adipophilin; adipose differentiation-related protein
UniProt Protein Name:Perilipin-2
UniProt Synonym Protein Names:Adipophilin; Adipose differentiation-related protein; ADRP
UniProt Gene Name:PLIN2  
UniProt Entry Name:PLIN2_HUMAN
Anti-PLIN2 Antibody (CAB6276)Western blot analysis of extracts of HepG2 cells, using PLIN2 antibody (CAB6276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-PLIN2 Antibody (CAB6276)Western blot analysis of extracts of various cell lines, using PLIN2 antibody (CAB6276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-PLIN2 Antibody (CAB6276)Western blot analysis of extracts of Rat heart, using PLIN2 antibody (CAB6276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PLIN2 Antibody (CAB6276)Immunohistochemistry of paraffin-embedded rat liver using PLIN2 Rabbit pAb (CAB6276) at dilution of 1:50 (40x lens).
Anti-PLIN2 Antibody (CAB6276)Immunohistochemistry of paraffin-embedded human liver using PLIN2 Rabbit pAb (CAB6276) at dilution of 1:50 (40x lens).
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