Cell Biology Antibodies 10
Anti-PLEK Antibody (CAB6305)
- SKU:
- CAB6305
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-PLEK Antibody |
| Antibody SKU: | CAB6305 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human PLEK (NP_002655.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, U-937, Mouse lung, Mouse spleen, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human PLEK (NP_002655.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEPK RIRE GYLV KKGS VFNT WKPM WVVL LEDG IEFY KKKS DNSP KGMI PLKG STLT SPCQ DFGK RMFV FKIT TTKQ QDHF FQAA FLEE RDAW VRDI KKAI KCIE GGQK FARK STRR SIRL PETI DLGA LYLS MKDT EKGI KELN LEKD KKIF NHCF TGNC VIDW LVSN QSVR NRQE GLMI ASSL LNEG YLQP AGDM SKSA VDGT AENP FLDN PDAF YYFP DSGF FCEE NSSD DDVI LKEE FRGV IIKQ GCLL KQGH RRKN WKVR KFIL REDP AYLH YYDP AGAE DPLG AIHL RGCV VTSV ESNS NGRK SEEE NLFE IITA DEVH YFLQ AATP KERT EWIR AIQM ASRT GK |
| Gene ID: | 5341 |
| Uniprot: | P08567 |
| Cellular Location: | |
| Calculated MW: | 40kDa |
| Observed MW: | 40kDa |
| Synonyms: | PLEK, P47 |
| Background: |
| UniProt Protein Function: | pleckstrin: Major protein kinase C substrate of platelets. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 2p13.3 Cellular Component: membrane; cytoplasm; extracellular region; cytosol Molecular Function:protein binding; protein homodimerization activity; protein kinase C binding; phosphatidylinositol-3,4-bisphosphate binding Biological Process: negative regulation of calcium-mediated signaling; integrin-mediated signaling pathway; platelet activation; phospholipase C inhibition; positive regulation of actin filament depolymerization; negative regulation of G-protein coupled receptor protein signaling pathway; positive regulation of integrin activation; platelet degranulation; cell projection organization and biogenesis; actin cytoskeleton reorganization; ruffle organization and biogenesis; positive regulation of actin filament bundle formation; hemopoietic progenitor cell differentiation; phosphatidylinositol metabolic process; cortical actin cytoskeleton organization and biogenesis; blood coagulation; vesicle docking during exocytosis |
| UniProt Code: | P08567 |
| NCBI GenInfo Identifier: | 317373523 |
| NCBI Gene ID: | 5341 |
| NCBI Accession: | P08567.3 |
| UniProt Secondary Accession: | P08567,Q53SU8, Q6FGM8, Q6FGQ1, Q8WV81, B2R9E8, |
| UniProt Related Accession: | P08567 |
| Molecular Weight: | 350 |
| NCBI Full Name: | Pleckstrin |
| NCBI Synonym Full Names: | pleckstrin |
| NCBI Official Symbol: | PLEK |
| NCBI Official Synonym Symbols: | P47 |
| NCBI Protein Information: | pleckstrin; platelet 47 kDa protein |
| UniProt Protein Name: | Pleckstrin |
| UniProt Synonym Protein Names: | Platelet 47 kDa protein; p47 |
| Protein Family: | Pleckstrin |
| UniProt Gene Name: | PLEK |
| UniProt Entry Name: | PLEK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using PLEK antibody (CAB6305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat heart using PLEK antibody (CAB6305) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using PLEK antibody (CAB6305) at dilution of 1:200 (40x lens). |
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