Antibody Name:	PIWIL1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB2150
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of mouse PIWIL1

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Mouse liver, Rat testis

Immunogen:	Recombinant protein of mouse PIWIL1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	57749
Uniprot:	Q9JMB7
Cellular Location:	Cytoplasm
Calculated MW:	95kDa/98kDa
Observed MW:	102kDa/110kDa

Synonyms:

PIWIL1, CT80.1, HIWI, MIWI, PIWI

Background:

This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PIWIL1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Membrane protein, peripheralCellular Component: polysome; mRNA cap complex; cytoplasmMolecular Function: mRNA binding; protein binding; single-stranded RNA binding; nucleic acid binding; RNA binding; protein kinase bindingBiological Process: regulation of translation; meiotic cell cycle; multicellular organismal development; RNA-mediated gene silencing; spermatogenesis; cell differentiation; spermatid development
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9JMB7
NCBI GenInfo Identifier:	81868808
NCBI Gene ID:	57749
NCBI Accession:	Q9JMB7.1
UniProt Secondary Accession:	Q9JMB7,Q6NXX0, A1L324, A1L325
UniProt Related Accession:	Q9JMB7
Molecular Weight:	862
NCBI Full Name:	Piwi-like protein 1
NCBI Synonym Full Names:	piwi-like RNA-mediated gene silencing 1
NCBI Official Symbol:	Piwil1
NCBI Official Synonym Symbols:	MIWI
NCBI Protein Information:	piwi-like protein 1; piwi like homolog 1; piwi-like homolog 1
UniProt Protein Name:	Piwi-like protein 1
UniProt Synonym Protein Names:
Protein Family:
UniProt Gene Name:	Piwil1
UniProt Entry Name:	PIWL1_MOUSE

	Western blot analysis of extracts of various cell lines, using PIWIL1 antibody (CAB2150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat testis using Piwil1 antibody (CAB2150) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using Piwil1 antibody (CAB2150) at dilution of 1:100 (40x lens).