Antibody Name:	Anti-PIP4K2A Antibody
Antibody SKU:	CAB7446
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-350 of human PIP4K2A (NP_005019.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, MCF7, Mouse brain, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-350 of human PIP4K2A (NP_005019.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APLP NDSQ ARSG ARFH TSYD KRYI IKTI TSED VAEM HNIL KKYH QYIV ECHG ITLL PQFL GMYR LNVD GVEI YVIV TRNV FSHR LSVY RKYD LKGS TVAR EASD KEKA KELP TLKD NDFI NEGQ KIYI DDNN KKVF LEKL KKDV EFLA QLKL MDYS LLVG IHDV ERAE QEEV ECEE NDGE EEGE SDGT HPVG TPPD SPGN TLNS SPPL APGE FDPN IDVY GIKC HENS PRK
Gene ID:	5305
Uniprot:	P48426
Cellular Location:	Cell membrane, Cytoplasm, Nucleus
Calculated MW:	39kDa/46kDa
Observed MW:	46kDa

Synonyms:

PIP4K2A, PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK

Background:

Phosphatidylinositol-5, 4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5, 4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.

UniProt Protein Function:	PIP4K2A: Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.
UniProt Protein Details:	Protein type:Kinase, lipid; EC 2.7.1.149; Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate Chromosomal Location of Human Ortholog: 10p12.2 Cellular Component: plasma membrane; nucleus; cytosol Molecular Function:1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; ATP binding Biological Process: phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process
NCBI Summary:	Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq, Jul 2008]
UniProt Code:	P48426
NCBI GenInfo Identifier:	18266879
NCBI Gene ID:	5305
NCBI Accession:	P48426.2
UniProt Secondary Accession:	P48426,P53807, Q5VUX3, B0YJ66, B4DGX2, D3DRV1,
UniProt Related Accession:	P48426
Molecular Weight:	406
NCBI Full Name:	Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
NCBI Synonym Full Names:	phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
NCBI Official Symbol:	PIP4K2A
NCBI Official Synonym Symbols:	PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
NCBI Protein Information:	phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; PIP5KIII; PIP5KIIalpha; PIP4KII-alpha; PI(5)P 4-kinase type II alpha; PtdIns(4)P-5-kinase B isoform; ptdIns(4)P-5-kinase C isoform; diphosphoinositide kinase 2-alpha; ptdIns(5)P-4-kinase isoform 2-alpha; 1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-4-phosphate-5-kinase; 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; phosphatidylinositol-5-phosphate 4-kinase type-2 alpha; phosphatidylinositol 5-phosphate 4-kinase type II alpha; phosphatidylinositol-4-phosphate 5-kinase, type II, alpha; type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform
UniProt Protein Name:	Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
UniProt Synonym Protein Names:	1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; PIP5KIII; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; PI(5)P 4-kinase type II alpha; PIP4KII-alpha; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PtdIns(5)P-4-kinase isoform 2-alpha
Protein Family:	Phosphatidylinositol 5-phosphate 4-kinase
UniProt Gene Name:	PIP4K2A
UniProt Entry Name:	PI42A_HUMAN

	Western blot analysis of extracts of various cell lines, using PIP4K2A antibody (CAB7446) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PIP4K2A antibody (CAB7446) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using PIP4K2A antibody (CAB7446) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using PIP4K2A antibody (CAB7446).