Antibody Name:	Anti-PHOX2B Antibody
Antibody SKU:	CAB9685
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PHOX2B (NP_003915.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, THP-1, HeLa, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human PHOX2B (NP_003915.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MYKM EYSY LNSS AYES CMAG MDTS SLAS AYAD FSSC SQAS GFQY NPIR TTFG ATSG CPSL TPGS CSLG TLRD HQSS PYAA VPYK LFTD HG
Gene ID:	8929
Uniprot:	Q99453
Cellular Location:	Nucleus
Calculated MW:	31kDa
Observed MW:	32kDa

Synonyms:

PHOX2B, NBLST2, NBPhox, PMX2B

Background:

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome.

UniProt Protein Function:	PHOX2B: Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum- response element. Defects in PHOX2B are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. Most mutations consist of 5-10 alanine expansions in the poly-Ala region from amino acids 241-260. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Defects in PHOX2B are the cause of susceptibility to neuroblastoma type 2 (NBLST2). A common neoplasm of early childhood arising from embryonic cells that form the primitive neural crest and give rise to the adrenal medulla and the sympathetic nervous system. Belongs to the paired homeobox family.
UniProt Protein Details:	Protein type:Transcription regulation; DNA-binding Chromosomal Location of Human Ortholog: 4p12 Cellular Component: nuclear chromatin Biological Process: autonomic nervous system development; cell differentiation in hindbrain; efferent axon development in a lateral line nerve; enteric nervous system development; glial cell differentiation; hindbrain tangential cell migration; inner ear development; neurological control of breathing; neuron migration; parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; regulation of gene expression; rhombencephalic reticular formation development; sympathetic nervous system development Disease: Central Hypoventilation Syndrome, Congenital; Neuroblastoma, Susceptibility To, 2
NCBI Summary:	The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome. [provided by RefSeq, Jul 2016]
UniProt Code:	Q99453
NCBI GenInfo Identifier:	116242712
NCBI Gene ID:	8929
NCBI Accession:	Q99453.2
UniProt Secondary Accession:	Q99453,Q6PJD9,
UniProt Related Accession:	Q99453
Molecular Weight:	31,621 Da
NCBI Full Name:	Paired mesoderm homeobox protein 2B
NCBI Synonym Full Names:	paired like homeobox 2b
NCBI Official Symbol:	PHOX2B
NCBI Official Synonym Symbols:	PMX2B; NBLST2; NBPhox
NCBI Protein Information:	paired mesoderm homeobox protein 2B
UniProt Protein Name:	Paired mesoderm homeobox protein 2B
UniProt Synonym Protein Names:	Neuroblastoma Phox; NBPhox; PHOX2B homeodomain protein; Paired-like homeobox 2B
Protein Family:	Paired mesoderm homeobox protein
UniProt Gene Name:	PHOX2B
UniProt Entry Name:	PHX2B_HUMAN

	Western blot analysis of extracts of various cell lines, using PHOX2B antibody (CAB9685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat liver using PHOX2B antibody (CAB9685) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using PHOX2B antibody (CAB9685) at dilution of 1:100 (40x lens).