Cell Biology Antibodies 16
Anti-Phospho-VASP-S157 pAb Antibody (CABP0763)
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- SKU:
- CABP0763
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-VASP-S157 Antibody |
| Antibody SKU: | CABP0763 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431 |
| Immunogen: | A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RVSN A |
| Gene ID: | 7408 |
| Uniprot: | P50552 |
| Cellular Location: | Cell junction, Cell projection, Cytoplasm, cytoskeleton, filopodium membrane, focal adhesion, lamellipodium membrane, tight junction |
| Calculated MW: | 39kDa |
| Observed MW: | 50kDa |
 | Western blot analysis of extracts of A431 cells, using Phospho-VASP-S157 antibody (CABP0763) at 1:2000 dilution. A431 cells were treated by Forskolin (10uM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat brain using Phospho-VASP-S157 antibody (CABP0763) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using Phospho-VASP-S157 antibody (CABP0763) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using Phospho-VASP-S157 antibody (CABP0763) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Anti-Phospho-VASP-S157 pAb (CABP0763). Western blot was performed from the immunoprecipitate using Anti-Phospho-VASP-S157 pAb (CABP0763) at a dilition of 1:1000. A-431 cells were treated by Forskolin (10 uM) at 37'C for 30 minutes after serum-starvation overnight. |
| Synonyms: | VASP |
| Background: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
| UniProt Protein Function: | VASP: vasodilator-stimulated phosphoprotein. Actin- and profilin-binding microfilament-associated protein. The phosphorylation of VASP is dynamically regulated by cellular adhesion to extracellular matrix. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19q13.32 Cellular Component: filopodium membrane; tight junction; focal adhesion; cytoplasm; plasma membrane; cytosol; actin cytoskeleton Molecular Function:protein binding; actin binding; SH3 domain binding; profilin binding Biological Process: axon guidance; positive regulation of actin filament polymerization; actin polymerization and/or depolymerization; neural tube closure; T cell receptor signaling pathway; protein homotetramerization |
| NCBI Summary: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P50552 |
| NCBI GenInfo Identifier: | 1718079 |
| NCBI Gene ID: | 7408 |
| NCBI Accession: | P50552.3 |
| UniProt Secondary Accession: | P50552,Q6PIZ1, Q93035, B2RBT9, |
| UniProt Related Accession: | P50552 |
| Molecular Weight: | 380 |
| NCBI Full Name: | Vasodilator-stimulated phosphoprotein |
| NCBI Synonym Full Names: | vasodilator-stimulated phosphoprotein |
| NCBI Official Symbol: | VASP |
| NCBI Protein Information: | vasodilator-stimulated phosphoprotein |
| UniProt Protein Name: | Vasodilator-stimulated phosphoprotein |
| Protein Family: | Vasodilator-stimulated phosphoprotein |
| UniProt Gene Name: | VASP |
| UniProt Entry Name: | VASP_HUMAN |
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