Cell Biology Antibodies 16

Phospho-Src-Y529 Rabbit Polyclonal Antibody (CABP0185)

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SKU:
CABP0185
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
ASV, SRC1, THC6, c-SRC, p60-Src, Phospho-Src-Y529
Research Use:
Cell Biology
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Description

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Phospho-Src-Y529 Antibody (CABP0185)

The Phospho-Src-Y529 Antibody (CABP0185) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and specifically binds to phosphorylated Src at residue Y529, allowing for precise detection and analysis of this important post-translational modification.Phosphorylation of Src at Y529 is known to regulate its activity and downstream signaling pathways, making it a critical player in various cellular processes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Phospho-Src-Y529 Antibody
SKU:CABP0185
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:EPQY Q
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:100
IF/ICC1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ASV, SRC1, THC6, c-SRC, p60-Src, Phospho-Src-Y529
Positive Sample:A-431 treated with EGF
Cellular Localization:Cell Membrane, Cytoplasm, Mitochondrion Inner Membrane, Nucleus, Cytoskeleton.
Calculated MW:60kDa
Observed MW:60kDa

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Purification MethodAffinity purification
Gene ID6714
RRIDAB_2771557
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit pAb (CABP0185) at 1:1000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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