Epigenetics & Nuclear Signaling Antibodies 5

Phospho-Smad2-T220 Rabbit Polyclonal Antibody (CABP0909)

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SKU:
CABP0909
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
JV18, LDS6, CHTD8, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, Phospho-Smad2-T220
Research Use:
Epigenetics and Nuclear Signaling
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Description

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Phospho-Smad2-T220 Antibody (CABP0909)

The Phospho-Smad2-T220 Antibody (CABP0909) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for the phosphorylated form of SMAD2 at threonine 220 and is validated for use in Western blot applications. By targeting the phosphorylated form of SMAD2, researchers can track the activation of the TGF-beta pathway in various cell types and investigate its role in diseases such as cancer, fibrosis, and immune disorders.SMAD2 is a critical mediator of TGF-beta signaling, playing a central role in regulating cell growth, differentiation, and apoptosis.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Phospho-Smad2-T220 Antibody
SKU:CABP0909
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:PETP P
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:JV18, LDS6, CHTD8, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2, Phospho-Smad2-T220
Positive Sample:HeLa treated with PMA
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:52kDa
Observed MW:60kDa

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

Purification MethodAffinity purification
Gene ID4087
RRIDAB_2863822
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HeLa cells using Phospho-Smad2-T220 Rabbit pAb (CABP0909) at 1:500 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast using Phospho-Smad2-T220 Rabbit pAb (CABP0909) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-Smad2-T220 Rabbit pAb (CABP0909) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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