Immunology Antibodies 3

Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit Polyclonal Antibody (CABP0827)

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SKU:
CABP0827
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1, Phospho-PLC gamma 1 (PLCG1)-S1248
Research Use:
Immunology
Frequently bought together:

Description

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Phospho-PLC gamma 1 (PLCG1)-S1248 Antibody (CABP0827)

The Phospho-PLC gamma 1 (PLCG1)-S1248 Antibody (CABP0827) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to the phosphorylated form of PLCG1, allowing for detection and analysis in a variety of cell types.PLCG1 is a key enzyme in the phosphoinositide signaling pathway, playing a critical role in cellular responses to various extracellular stimuli. Phosphorylation at serine 1248 has been implicated in regulating PLCG1 activity and downstream signaling events.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Phospho-PLC gamma 1 (PLCG1)-S1248 Antibody
SKU:CABP0827
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:REGS F
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:10 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1, Phospho-PLC gamma 1 (PLCG1)-S1248
Positive Sample:Jurkat treated with PMA
Cellular Localization:Cell Projection, Lamellipodium, Ruffle.
Calculated MW:149kDa
Observed MW:149kDa

The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID5335
RRIDAB_2771432
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Jurkat cells, using Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit pAb (CABP0827) at dilution or PLC gamma 1 (PLCG1) antibody (CAB15704). Jurkat cells were treated with PMA/TPA (200 nM) at 37℃ for 10 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit pAb (CABP0827) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
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