Epigenetics & Nuclear Signaling Antibodies 4

Anti-Phospho-PDPK1-S241 Antibody (CABP0426)

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SKU:
CABP0426

Description

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Antibody Name:Anti-Phospho-PDPK1-S241 Antibody
Antibody SKU:CABP0426
Antibody Size:50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding S241 of human PDPK1
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293
Immunogen:A phospho specific peptide corresponding to residues surrounding S241 of human PDPK1
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5170
Uniprot:O15530
Cellular Location:Cell junction, Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein, focal adhesion
Calculated MW:48kDa/50kDa/58kDa/60kDa/63kDa
Observed MW:63kDa
Anti-Phospho-PDPK1-S241 Antibody (CABP0426)Western blot analysis of extracts from 293 cells, using Phospho-PDPK1-S241 antibody (CABP0426). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Anti-Phospho-PDPK1-S241 Antibody (CABP0426)Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-PDPK1-S241 antibody (CABP0426).
Anti-Phospho-PDPK1-S241 Antibody (CABP0426)Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-PDPK1-S241 antibody (CABP0426).
Synonyms:PDPK1, PDK1, PDPK2, PDPK2P, PRO0461
Background:Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.
UniProt Protein Function:Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.
UniProt Code:O15530
NCBI GenInfo Identifier:17380162
NCBI Gene ID:5170
NCBI Accession:O15530.1
UniProt Secondary Accession:O15530,Q59EH6, Q6FI20, Q8IV52, Q9BRD5, H0Y4Z0,
UniProt Related Accession:O15530,Q6A1A2
Molecular Weight:50,838 Da
NCBI Full Name:3-phosphoinositide-dependent protein kinase 1
NCBI Synonym Full Names:3-phosphoinositide dependent protein kinase 1
NCBI Official Symbol:PDPK1  
NCBI Official Synonym Symbols:PDK1; PDPK2; PDPK2P; PRO0461  
NCBI Protein Information:3-phosphoinositide-dependent protein kinase 1
UniProt Protein Name:3-phosphoinositide-dependent protein kinase 1
Protein Family:3-phosphoinositide-dependent protein kinase
UniProt Gene Name:PDPK1  
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Additional Information

Product Type:
Antibody
Applications:
WB
Applications:
IHC
Applications:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
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