Phospho-p53-T81 Rabbit Polyclonal Antibody (CABP1253)

(No reviews yet) Write a Review
SKU:
CABP1253
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Reactivity:
Human, Rat
Synonyms:
P53, BCC7, LFS1, BMFS5, TRP53, Phospho-p53-T81
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Phospho-p53-T81 Antibody (CABP1253)

The Phospho-p53-T81 Antibody (CABP1253) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Phosphorylation of p53 at threonine 81 has been linked to the activation of p53 in response to DNA damage and cellular stress, leading to the induction of cell cycle arrest, apoptosis, and DNA repair pathways.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:Phospho-p53-T81 Antibody
SKU:CABP1253
Size:20μL, 100μL
Reactivity:Human, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:APTP A
Tested Applications:WB ELISA
Recommended Dilution:
WB1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:P53, BCC7, LFS1, BMFS5, TRP53, Phospho-p53-T81
Positive Sample:C6 treated with nocodazole
Cellular Localization:Cytoplasm, Endoplasmic Reticulum, Mitochondrion Matrix, Nucleus, Pml Body.
Calculated MW:44kDa
Observed MW:53kDa

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Purification MethodAffinity purification
Gene ID7157
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-p53-T81 Rabbit pAb (CABP1253) at 1:500 dilution. C6 cells were treated with nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
View AllClose

0 Reviews

View AllClose

Related Products