Developmental Biology

Phospho-NEDD4L-S448 Rabbit Polyclonal Antibody (CABP0843)

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SKU:
CABP0843
Product Type:
Antibody
Applications:
WB
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
RSP5, PVNH7, NEDD4-2, NEDD4.2, hNEDD4-2, Phospho-NEDD4L-S448
Research Use:
Developmental Biology
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Description

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Phospho-NEDD4L-S448 Antibody (CABP0843)

The Phospho-NEDD4L-S448 Antibody (CABP0843) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the phosphorylated form of Nedd4L at Serine 448 and has been validated for use in multiple applications, including Western blot and immunohistochemistry.Nedd4L plays a significant role in regulating protein degradation and trafficking, particularly in the context of cell growth, differentiation, and survival. Phosphorylation of Nedd4L at Serine 448 has been implicated in various cellular processes, making this antibody essential for investigating the mechanism of action and functional consequences of this post-translational modification.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Phospho-NEDD4L-S448 Antibody
SKU:CABP0843
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:SLSS P
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RSP5, PVNH7, NEDD4-2, NEDD4.2, hNEDD4-2, Phospho-NEDD4L-S448
Positive Sample:293T, NIH/3T3, C6
Cellular Localization:Cytoplasm.
Calculated MW:112kDa
Observed MW:100kDa/130kDa

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification MethodAffinity purification
Gene ID23327
RRIDAB_2771357
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (CABP0843) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Product ImageWestern blot analysis of lysates from 293T cells, using Phospho-NEDD4L-S448 Rabbit pAb (CAB8085). 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
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