Developmental Biology
Anti-Phospho-NEDD4L-S448 pAb Antibody (CABP0843)
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- SKU:
- CABP0843
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-Phospho-NEDD4L-S448 Antibody |
| Antibody SKU: | CABP0843 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, NIH/3T3, C6 |
| Immunogen: | A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SLSS P |
| Gene ID: | 23327 |
| Uniprot: | Q96PU5 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 96-111kDa |
| Observed MW: | 100kDa/130kDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-NEDD4L-S448 antibody (CABP0843) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Western blot analysis of extracts of 293T cells, using Anti-Phospho-NEDD4L-S448 pAb (CABP0843) at 1:1000 dilution or NEDD4L antibody (CAB8085).293T cells were treated by CIP(20uL/400ul) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
| Synonyms: | NEDD4L, NEDD4-2, NEDD4.2, PVNH7, RSP5, hNEDD4-2 |
| Background: | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | NEDD4L: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Interacts with UBE2E3. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with Epstein-Barr virus LMP2A. Interacts with TNK2. Interacts with WNK1. Interacts with SGK1. By androgens in prostate, and by albumin in kidney. Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Activated by NDFIP1- and NDFIP2-binding. 9 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 18q21.31 Cellular Component: cytoplasm; cytosol; intracellular; nucleoplasm; nucleus; plasma membrane Molecular Function:potassium channel inhibitor activity; potassium channel regulator activity; protein binding; sodium channel inhibitor activity; sodium channel regulator activity; ubiquitin-protein ligase activity Biological Process: negative regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of membrane potential; response to metal ion; viral infectious cycle |
| NCBI Summary: | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| UniProt Code: | Q96PU5 |
| NCBI GenInfo Identifier: | 73921204 |
| NCBI Gene ID: | 23327 |
| NCBI Accession: | Q96PU5.2 |
| UniProt Secondary Accession: | Q96PU5,O43165, Q3LSM7, Q7Z5F1, Q7Z5F2, Q7Z5N3, Q8N5A7 Q8WUU9, Q9BW58, Q9H2W4, Q9NT88, |
| UniProt Related Accession: | Q96PU5 |
| Molecular Weight: | 96,271 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase NEDD4-like |
| NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase |
| NCBI Official Symbol: | NEDD4L |
| NCBI Official Synonym Symbols: | RSP5; NEDD4-2; NEDD4.2; hNEDD4-2 |
| NCBI Protein Information: | E3 ubiquitin-protein ligase NEDD4-like |
| UniProt Protein Name: | E3 ubiquitin-protein ligase NEDD4-like |
| UniProt Synonym Protein Names: | NEDD4.2; Nedd4-2 |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | NEDD4L |
| UniProt Entry Name: | NED4L_HUMAN |
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